Photonic crystal fiber-based immunosensor for high-performance detection of alpha fetoprotein.

Journal: Biosensors & bioelectronics
PMID: 28063391

Abstract

We have developed a sensitive photonic crystal fiber (PCF)-based immunosensor for detection of alpha fetoprotein (AFP). The unique PCF possesses a morphology characterized by numerous pore structures and a large surface area-to-volume ratio, which can be used as an immune-reaction carrier to improve the sensitivity and reaction speed of AFP detection. The PCF-based immunosensor possesses a low limit of detection of 0.1ng/mL, which is five times lower than that of the capillary-based sensor and 35 times lower than that of the traditional enzyme-linked immunosorbent assay. The wide linear dynamic range of 0.1-150ng/mL makes the developed immunosensor suitable for clinical practice. The proposed method was successfully applied to AFP detection in a clinical serum sample with acceptable precision. It is indicated that the present PCF-based immunosensor could be used as an attractive analytical platform for sensitive and specific detection of cancer biomarkers.

Authors

  • Xiaoxia Liu
  • Xingda Song
    Key Laboratory of Nanobiosensing and Nanobioanalysis at Universities of Jilin Province, Department of Chemistry, Northeast Normal University, 5268 Renmin Street, Changchun, Jilin Province 130024, PR China.
  • Zhiyong Dong
    Departments of Histology and Embryology, Basic Medical College, Jilin University, Changchun 130021, PR China.
  • Xiaoting Meng
    Departments of Histology and Embryology, Basic Medical College, Jilin University, Changchun 130021, PR China.
  • Yiping Chen
    Beijing Engineering Research Center for BioNanotechnology & CAS Key Laboratory for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Beijing 100190, PR China. Electronic address: chenyp@nanoctr.cn.
  • Li Yang
    Department of Pharmacy, Beijing Tiantan Hospital, Capital Medical University, Beijing, China.

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