Comparison of Droplet Digital PCR and Real Time PCR Method for HBV DNA Quantification.
Journal:
Journal of the Medical Association of Thailand = Chotmaihet thangphaet
PMID:
26817223
Abstract
BACKGROUND: HBV infection causes a potential serious public health problem. The ability to detect HBV DNA concentration is an important issue that had been continuously improved. When using quantitative polymerase chain reaction (qPCR), several factors are of concern, for example, sources of material, standard curve calibration, and PCR efficiency. Digital PCR (dPCR) is an alternative PCR-based technique for absolute quantification using Poisson's statistics without requiring a standard curve.