Measurement-oriented deep-learning workflow for improved segmentation of myelin and axons in high-resolution images of human cerebral white matter.

Journal: Journal of neuroscience methods
Published Date: Oct 1, 2019

Abstract

BACKGROUND: Standard segmentation of high-contrast electron micrographs (EM) identifies myelin accurately but does not translate easily into measurements of individual axons and their myelin, even in cross-sections of parallel fibers. We describe automated segmentation and measurement of each myelinated axon and its sheath in EMs of arbitrarily oriented human white matter from autopsies.

Authors

  • Predrag Janjic
    Research Center for Computer Science and Information Technology, Macedonian Academy of Sciences and Arts, Bul. Krste Misirkov 2, 1000, Skopje, North Macedonia. Electronic address: predrag.a.janjic@gmail.com.
  • Kristijan Petrovski
    Research Center for Computer Science and Information Technology, Macedonian Academy of Sciences and Arts, Bul. Krste Misirkov 2, 1000, Skopje, North Macedonia.
  • Blagoja Dolgoski
    Institute of Pathology, School of Medicine, Ss. Cyril and Methodius University Skopje, ul. 50ta Divizija 6, 1000, Skopje, North Macedonia.
  • John Smiley
    Nathan S. Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, NY 10962, USA.
  • Panche Zdravkovski
    Institute of Pathology, School of Medicine, Ss. Cyril and Methodius University Skopje, ul. 50ta Divizija 6, 1000, Skopje, North Macedonia.
  • Goran Pavlovski
    Institute of Forensic Medicine, School of Medicine, Ss. Cyril and Methodius University Skopje, ul. 50ta Divizija 6, 1000, Skopje, North Macedonia.
  • Zlatko Jakjovski
    Institute of Forensic Medicine, School of Medicine, Ss. Cyril and Methodius University Skopje, ul. 50ta Divizija 6, 1000, Skopje, North Macedonia.
  • Natasa Davceva
    Institute of Forensic Medicine, School of Medicine, Ss. Cyril and Methodius University Skopje, ul. 50ta Divizija 6, 1000, Skopje, North Macedonia.
  • Verica Poposka
    Institute of Forensic Medicine, School of Medicine, Ss. Cyril and Methodius University Skopje, ul. 50ta Divizija 6, 1000, Skopje, North Macedonia.
  • Aleksandar Stankov
    Institute of Forensic Medicine, School of Medicine, Ss. Cyril and Methodius University Skopje, ul. 50ta Divizija 6, 1000, Skopje, North Macedonia.
  • Gorazd Rosoklija
    Department of Psychiatry, Columbia University, New York, USA; Division of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, 1051 Riverside Drive, Unit 42, New York, NY, 10032, USA; Macedonian Academy of Sciences and Arts, Bul. Krste Misirkov 2, 1000, Skopje, North Macedonia.
  • Gordana Petrushevska
    Institute of Pathology, School of Medicine, Ss. Cyril and Methodius University Skopje, ul. 50ta Divizija 6, 1000, Skopje, North Macedonia.
  • Ljupco Kocarev
    Macedonian Academy of Sciences and Arts, Research Center for Computer Science and Information Technologies, Skopje, 1000, Republic of Macedonia.
  • Andrew J Dwork
    Department of Psychiatry, Columbia University, New York, USA; Division of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, 1051 Riverside Drive, Unit 42, New York, NY, 10032, USA; Macedonian Academy of Sciences and Arts, Bul. Krste Misirkov 2, 1000, Skopje, North Macedonia; Department of Pathology and Cell Biology, Columbia University, New York, USA.

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