Clinical Significance of Circulating Cardiomyocyte-Specific Cell-Free DNA in Patients With Heart Failure: A Proof-of-Concept Study.

Journal: The Canadian journal of cardiology
PMID: 32001048

Abstract

We investigated clinical significance of cell-free DNA (cfDNA) in heart failure. This study enrolled 32 heart failure patients and 28 control subjects. Total cfDNA levels were not different between groups (P = 0.343). Bisulfite-digital polymerase chain reaction using the unmethylated FAM101A locus demonstrated that cardiomyocyte-specific cfDNA was significantly elevated in heart failure patients compared with control subjects (median 0.99 [interquartile range 0.77-1.98] vs 0 [0-0.91] copies/mL; P = 0.003). Cardiomyocyte-specific cfDNA significantly discriminated heart failure patients from control subjects (area under the receiver operating characteristic curve, 0.716; P = 0.003) and was positively correlated with troponin I (r = 0.438; P = 0.003) but not with B-type natriuretic peptide (r = 0.275; P = 0.058). cfDNA may be a novel biomarker to measure cardiomyocyte death in heart failure.

Authors

  • Tetsuro Yokokawa
    Department of Cardiovascular Medicine, Fukushima Medical University, Fukushima, Japan; Department of Pulmonary Hypertension, Fukushima Medical University, Fukushima, Japan.
  • Tomofumi Misaka
    Department of Cardiovascular Medicine, Fukushima Medical University, Fukushima, Japan; Department of Advanced Cardiac Therapeutics, Fukushima Medical University, Fukushima, Japan. Electronic address: misaka83@fmu.ac.jp.
  • Yusuke Kimishima
    Department of Cardiovascular Medicine, Fukushima Medical University, Fukushima, Japan.
  • Takeshi Shimizu
    Department of Cardiovascular Medicine, Fukushima Medical University, Fukushima, Japan.
  • Takashi Kaneshiro
    Department of Cardiovascular Medicine, Fukushima Medical University, Fukushima, Japan; Department of Arrhythmia and Cardiac Pacing, Fukushima Medical University, Fukushima, Japan.
  • Yasuchika Takeishi
    Department of Cardiovascular Medicine, Fukushima Medical University, Fukushima, Japan.

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