Desert actinobacteria as a source of bioactive compounds production with a special emphases on Pyridine-2,5-diacetamide a new pyridine alkaloid produced by Streptomyces sp. DA3-7.

Journal: Microbiological research
PMID: 29458846

Abstract

In the present study, 134 morphologically distinct actinobacteria isolates were obtained from soil samples from 10 different localities in the Saudi Arabian desert. The preliminary screening revealed that 16 of these isolates possessed antimicrobial activity. One isolate, which was identified as Streptomyces sp. DA3-7, possessed broad-spectrum antimicrobial activity against both gram-positive and gram-negative bacteria, as well as against fungi, and modified nutrient glucose medium was suitable for Streptomyces sp. DA3-7 to produce extracellular metabolites. The ethyl acetate extract of Streptomyces sp. DA3-7 exhibited antimicrobial activity against Enterococcus faecalis and Salmonella typhimurium, with minimum inhibitory concentrations of 78 and 156μg/mL, respectively, as well as strong cytotoxicity (24h IC 85μg/mL) against MCF-7 human breast adenocarcinoma cells. The active compound was separated, purified, and identified as Pyridine-2,5-diacetamide (CHNO+H+, 194.21), which possessed a lowest minimum inhibitory concentration (31.25μg/mL) against both Escherichia coli and Cryptococcus neoformans. The antimicrobial activities of this novel compound are reported here for the first time.

Authors

  • Krishnasamy Nithya
    Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
  • Chinnasamy Muthukumar
    Department of Botany, National College (Autonomous), Tiruchirappalli 620001, Tamil Nadu, India.
  • Bhaskar Biswas
    Department of Chemistry, Surendranath College, Kolkata 700009, India.
  • Naiyf S Alharbi
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Shine Kadaikunnan
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Jamal M Khaled
    Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
  • Dharumadurai Dhanasekaran
    Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India; Department of Molecular, Cellular and Biomedical Sciences, University of New Hampshire, Durham, NH, 03824-2617, USA. Electronic address: dhansdd@gmail.com.

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