Deciphering dysregulation and CpG methylation in hepatocellular carcinoma using multi-omics and machine learning.

Journal: Epigenomics
Published Date:

Abstract

This study investigates the altered expression and CpG methylation patterns of histone demethylase in hepatocellular carcinoma (HCC), aiming to uncover insights and promising diagnostics biomarkers. Leveraging TCGA-LIHC multi-omics data, we employed R/Bioconductor libraries and Cytoscape to analyze and construct a gene correlation network, and LASSO regression to develop an HCC-predictive model. In HCC, downregulation is correlated with CpGs hypermethylation. Differential gene correlation analysis unveiled a liver carcinoma-associated network marked by increased cell division and compromised liver-specific functions. The LASSO regression identified a highly accurate HCC prediction signature, prominently featuring CpG methylation at cg02871891. Our study uncovers CpG hypermethylation at cg02871891, possibly influencing downregulation in HCC, suggesting these as promising biomarkers and targets.

Authors

  • Firoz Ahmed
    Department of Biological Sciences, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
  • Nitish Kumar Mishra
    Dept. of Genetics, Cell Biology & Anatomy, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68182, USA. nitish.mishra@unmc.edu.
  • Othman A Alghamdi
    Department of Biological Sciences, Collage of Science, University of Jeddah, Jeddah, Kingdom of Saudi Arabia.
  • Mohammad Imran Khan
    Research Center, King Faisal Specialist Hospital & Research Centre, Jeddah, Saudi Arabia.
  • Aamir Ahmad
    Translational Research Institute, Academic Health System, Hamad Medical Corporation, Doha, 3050, Qatar.
  • Nargis Khan
    Snyder Institute of Chronic Diseases, Health Research & Innovation Center, Cumming School of Medicine, University of Calgary, Alberta, Canada.
  • Mohammad Rehan
    Snyder Institute of Chronic Diseases, Health Research & Innovation Center, Cumming School of Medicine, University of Calgary, Alberta, Canada.