AIMC Topic: Cryoelectron Microscopy

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Comparing models and experimental structures of the GPR101 receptor: Artificial intelligence yields highly accurate models.

Journal of molecular graphics & modelling
Experimental structures solved through cryo-electron microscopy have recently been published for GPR101, a G protein-coupled receptor (GPCR) implicated in the genetic condition X-linked acrogigantism (X-LAG). Here, we compared these experimental stru...

Advancing Molecular Simulations: Merging Physical Models, Experiments, and AI to Tackle Multiscale Complexity.

The journal of physical chemistry letters
Proteins and protein complexes form adaptable networks that regulate essential biochemical pathways and define cell phenotypes through dynamic mechanisms and interactions. Advances in structural biology and molecular simulations have revealed how pro...

A review of denoising methods in single-particle cryo-EM.

Micron (Oxford, England : 1993)
Cryo-EM has become a vital technique for resolving macromolecular structures at near-atomic resolution, enabling the visualization of proteins and large molecular complexes. However, the images are often accompanied by extremely low SNR, which poses ...

Fitting Atomic Structures into Cryo-EM Maps by Coupling Deep Learning-Enhanced Map Processing with Global-Local Optimization.

Journal of chemical information and modeling
With the breakthroughs in protein structure prediction technology, constructing atomic structures from cryo-electron microscopy (cryo-EM) density maps through structural fitting has become increasingly critical. However, the accuracy of the construct...

The Evolving Landscape of Protein Allostery: From Computational and Experimental Perspectives.

Journal of molecular biology
Protein allostery is a fundamental biological regulatory mechanism that allows communication between distant locations within a protein, modifying its function in response to signals. Experimental techniques, such as NMR spectroscopy and cryo-electro...

FakET: Simulating cryo-electron tomograms with neural style transfer.

Structure (London, England : 1993)
In cryo-electron microscopy, accurate particle localization and classification are imperative. Recent deep learning solutions, though successful, require extensive training datasets. The protracted generation time of physics-based models, often emplo...

Advancing structure modeling from cryo-EM maps with deep learning.

Biochemical Society transactions
Cryo-electron microscopy (cryo-EM) has revolutionized structural biology by enabling the determination of biomolecular structures that are challenging to resolve using conventional methods. Interpreting a cryo-EM map requires accurate modeling of the...

AI-based methods for biomolecular structure modeling for Cryo-EM.

Current opinion in structural biology
Cryo-electron microscopy (Cryo-EM) has revolutionized structural biology by enabling the determination of macromolecular structures that were challenging to study with conventional methods. Processing cryo-EM data involves several computational steps...

Deciphering Protein Secondary Structures and Nucleic Acids in Cryo-EM Maps Using Deep Learning.

Journal of chemical information and modeling
With the resolution revolution of cryo-electron microscopy (cryo-EM) and the rapid development of image processing technology, cryo-EM has become an indispensable experimental method for determining the three-dimensional structures of biological macr...

Overcoming the preferred-orientation problem in cryo-EM with self-supervised deep learning.

Nature methods
While advances in single-particle cryo-EM have enabled the structural determination of macromolecular complexes at atomic resolution, particle orientation bias (the 'preferred' orientation problem) remains a complication for most specimens. Existing ...