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Protein Engineering

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Applications and challenges in designing VHH-based bispecific antibodies: leveraging machine learning solutions.

mAbs
The development of bispecific antibodies that bind at least two different targets relies on bringing together multiple binding domains with different binding properties and biophysical characteristics to produce a drug-like therapeutic. These buildin...

GraphKM: machine and deep learning for K prediction of wildtype and mutant enzymes.

BMC bioinformatics
Michaelis constant (K) is one of essential parameters for enzymes kinetics in the fields of protein engineering, enzyme engineering, and synthetic biology. As overwhelming experimental measurements of K are difficult and time-consuming, prediction of...

Security challenges by AI-assisted protein design : The ability to design proteins in silico could pose a new threat for biosecurity and biosafety.

EMBO reports
Scientists and security experts are concerned that the increasing power of AI-assisted protein design and synthesis could be abused by various actors for terrorist or criminal purposes. [Image: see text]

Generative artificial intelligence for de novo protein design.

Current opinion in structural biology
Engineering new molecules with desirable functions and properties has the potential to extend our ability to engineer proteins beyond what nature has so far evolved. Advances in the so-called 'de novo' design problem have recently been brought forwar...

Computational scoring and experimental evaluation of enzymes generated by neural networks.

Nature biotechnology
In recent years, generative protein sequence models have been developed to sample novel sequences. However, predicting whether generated proteins will fold and function remains challenging. We evaluate a set of 20 diverse computational metrics to ass...

Protein Engineering with Lightweight Graph Denoising Neural Networks.

Journal of chemical information and modeling
Protein engineering faces challenges in finding optimal mutants from a massive pool of candidate mutants. In this study, we introduce a deep-learning-based data-efficient fitness prediction tool to steer protein engineering. Our methodology establish...

A systematic analysis of regression models for protein engineering.

PLoS computational biology
To optimize proteins for particular traits holds great promise for industrial and pharmaceutical purposes. Machine Learning is increasingly applied in this field to predict properties of proteins, thereby guiding the experimental optimization process...

[Progress in the application of artificial intelligence-assisted molecular modification of enzymes].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Natural enzymes are often difficult to meet the needs of application and research in terms of activity, enantiomer selectivity or thermal stability. Therefore, it is an important task of enzyme engineering to explore efficient molecular modification ...

Machine learning in biological physics: From biomolecular prediction to design.

Proceedings of the National Academy of Sciences of the United States of America
Machine learning has been proposed as an alternative to theoretical modeling when dealing with complex problems in biological physics. However, in this perspective, we argue that a more successful approach is a proper combination of these two methodo...

Machine learning-aided engineering of a cytochrome P450 for optimal bioconversion of lignin fragments.

Physical chemistry chemical physics : PCCP
Using machine learning, molecular dynamics simulations, and density functional theory calculations we gain insight into the selectivity patterns of substrate activation by the cytochromes P450. In nature, the reactions catalyzed by the P450s lead to ...