Integrated functional neuronal network analysis of 3D silk-collagen scaffold-based mouse cortical culture.

Journal: STAR protocols
PMID: 33537680

Abstract

Bioengineered 3D tunable neuronal constructs are a versatile platform for studying neuronal network functions, offering numerous advantages over existing technologies and providing for the discovery of new biological insights. Functional neural networks can be evaluated using calcium imaging and quantitatively described using network science. This protocol includes instructions for fabricating protein-based composite scaffolds, 3D culture of embryonic mouse cortical neurons, virally induced expression of GCaMP6f, wide-field calcium imaging, and computational analysis with open-source software and custom MATLAB code. For complete details on the use and execution of this protocol, please refer to Dingle et al. (2020).

Authors

  • Yu-Ting L Dingle
    Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
  • Mattia Bonzanni
    Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
  • Volha Liaudanskaya
    Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
  • Thomas J F Nieland
    Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.
  • David L Kaplan
    Department of Biomedical Engineering, Tufts University, Medford, MA, 02155, USA.

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