Chemical characterization, antioxidant, genotoxic and in vitro cytotoxic activity assessment of Juniperus communis var. saxatilis.

Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
PMID: 29287791

Abstract

Chemical composition and antioxidative, genotoxic and cytotoxic potential of essential oil (EO) and post-distillation waste (PDW) of Serbian Juniperus communis L. var. saxatilis Pall. was studied in human lung carcinoma (A549) and normal lung fibroblast (MRC-5) cells. GC-MS analysis identified 93.95% of total EO content and determined α-pinen as a dominant component (23.61%). LC-MS/MS analysis of PDW pointed at rutin (12.2 mg g) and quinic acid (11.1 mg g) as the most abundant. Antioxidativity of PDW was strong in DPPH (IC was 5.27 μg mL), and moderate in TBA and FRAP assays. Both substances were more cytotoxic to A549 than to MRC-5 cells. Obtained IC values were 69.4 μg mL and 120 μg mL for EO, and 1.27 mg mL and 2.86 mg mL for PDW, respectively. PDW was genotoxic (0.3 mg mL and 1 mg mL in A549 and MRC-5 cells, respectively) and induced apoptosis and arrested cell cycle in G2/M phase in A549 cells (0.3 mg mL). In mixtures with doxorubicin cytotoxicity of EO and PDW increased, and combination index values (0.12-0.18) revealed clear synergistic effect, stronger in cancer cells. This indicates that J. communis var. saxatilis could decrease the chemotherapeutic doses of doxorubicin, potentially reducing its side effects.

Authors

  • Bojana Vasilijević
    Department of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: bojanav@bio.bg.ac.rs.
  • Jelena Knežević-Vukčević
    Department of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: jelenakv@bio.bg.ac.rs.
  • Dragana Mitić-Ćulafić
    Department of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: mdragana@bio.bg.ac.rs.
  • Dejan Orčić
    Department for Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia. Electronic address: dejan.orcic@dh.uns.ac.rs.
  • Marina Francišković
    Department for Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia. Electronic address: marina.franciskovic@dh.uns.ac.rs.
  • Tatjana Srdic-Rajic
    Department of Experimental Oncology, Institute for Oncology and Radiology of Serbia, Pasterova 14, 11000, Belgrade, Serbia. Electronic address: tsrdic@gmail.com.
  • Marina Jovanović
    Department of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: marina.rajic.jovanovic@gmail.com.
  • Biljana Nikolić
    Department of Microbiology, Center for Genotoxicology and Ecogenotoxicology, Faculty of Biology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia. Electronic address: biljanan@bio.bg.ac.rs.

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