Antioxidant and antiproliferative activities of protein hydrolysate from the rhizomes of Zingiberaceae plants.
Journal:
Pakistan journal of pharmaceutical sciences
PMID:
28375103
Abstract
Plant proteins have been investigated for their antioxidant activities, but there are still no reports detailing the antioxidant activity levels of plants in the Zingiberaceae family, which are popular food agents and used in folklore medicine. In this study, the crude rhizome protein extract and associated pepsin/pancreatin protein hydrolysate of 15 plants in the Zingiberaceae family were screened using the DPPH method for antioxidant activity. The protein hydrolysate of C. zedoaria possessed the highest antioxidant activity (ICof 25.7±6.3µg/mL), which was close to that of the reference ascorbic acid (ICof 22.3±1.8µg/mL). After enrichment by Q Sepharose ion exchange chromatography using a five step elution gradient of increasing NaCl concentration (0, 0.25, 0.5, 0.75 and 1M), the fraction eluting in the 0.5M NaCl (F50) showed the highest antioxidant activity (IC of 41.78±2.9µg/mL), and was found to have weak in vitro cytotoxicity against the HEP-G2 and SW620 cell lines (IC of 200.8±11.8 and 241.0±9.3µg/mL, respectively), but not the BT474, CHAGO and KATO-3 cell lines. F50 had an estimated molecular weight by MALDI-TOF mass spectrometry of 12,400-12,800 Da.
Authors
Keywords
Antineoplastic Agents, Phytogenic
Antioxidants
Ascorbic Acid
Biphenyl Compounds
Cell Proliferation
Chromatography, Ion Exchange
Dose-Response Relationship, Drug
Hep G2 Cells
Humans
Inhibitory Concentration 50
Molecular Weight
Neoplasms
Phytotherapy
Picrates
Plant Extracts
Plant Proteins
Plants, Medicinal
Protein Hydrolysates
Rhizome
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Zingiberaceae