Robotic Actuation-Mediated Quantitative Mechanogenetics for Noninvasive and On-Demand Cancer Therapy.

Journal: Advanced science (Weinheim, Baden-Wurttemberg, Germany)
Published Date: Mar 21, 2024

Abstract

Cell mechanotransduction signals are important targets for physical therapy. However, current physiotherapy heavily relies on ultrasound, which is generated by high-power equipment or amplified by auxiliary drugs, potentially causing undesired side effects. To address current limitations, a robotic actuation-mediated therapy is developed that utilizes gentle mechanical loads to activate mechanosensitive ion channels. The resulting calcium influx precisely regulated the expression of recombinant tumor suppressor protein and death-associated protein kinase, leading to programmed apoptosis of cancer cell line through caspase-dependent pathway. In stark contrast to traditional gene therapy, the complete elimination of early- and middle-stage tumors (volume ≤ 100 mm) and significant growth inhibition of late-stage tumor (500 mm) are realized in tumor-bearing mice by transfecting mechanogenetic circuits and treating daily with quantitative robotic actuation in a form of 5 min treatment over the course of 14 days. Thus, this massage-derived therapy represents a quantitative strategy for cancer treatment.

Authors

  • Yangyi Liu
    Center of Materials Science and Optoelectronics Engineering, College of Materials Science and Optoelectronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Jingjing Li
    Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China.
  • Yi Zhang
    Department of Thyroid Surgery, China-Japan Union Hospital of Jilin University, Jilin University, Changchun, China.
  • Fan Wang
    Faculty of Information Engineering and Automation, Kunming University of Science and Technology, Kunming, China.
  • Juanjuan Su
    Center of Materials Science and Optoelectronics Engineering, College of Materials Science and Optoelectronic Technology, University of Chinese Academy of Sciences, Beijing, 100049, China.
  • Chao Ma
  • Shuyi Zhang
    Department of Intensive Care Medicine, Renji Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China.
  • Yanan Du
    Department of Biomedical Engineering, School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, 100084, China.
  • Chunhai Fan
    Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, CAS Key Laboratory of Interfacial Physics and Technology, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
  • Hongjie Zhang
    Tianjin Key Laboratory of Modern Mechatronics Equipment Technology, School of Mechanical Engineering, Tiangong University, Tianjin 300387, China.
  • Kai Liu
    College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China.

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