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Binding Sites

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BIPSPI+: Mining Type-Specific Datasets of Protein Complexes to Improve Protein Binding Site Prediction.

Journal of molecular biology
Computational approaches for predicting protein-protein interfaces are extremely useful for understanding and modelling the quaternary structure of protein assemblies. In particular, partner-specific binding site prediction methods allow delineating ...

Predicting residues involved in anti-DNA autoantibodies with limited neural networks.

Medical & biological engineering & computing
Computer-aided rational vaccine design (RVD) and synthetic pharmacology are rapidly developing fields that leverage existing datasets for developing compounds of interest. Computational proteomics utilizes algorithms and models to probe proteins for ...

DeepCAGE: Incorporating Transcription Factors in Genome-wide Prediction of Chromatin Accessibility.

Genomics, proteomics & bioinformatics
Although computational approaches have been complementing high-throughput biological experiments for the identification of functional regions in the human genome, it remains a great challenge to systematically decipher interactions between transcript...

Data Mining Meets Machine Learning: A Novel ANN-based Multi-body Interaction Docking Scoring Function (MBI-score) Based on Utilizing Frequent Geometric and Chemical Patterns of Interfacial Atoms in Native Protein-ligand Complexes.

Molecular informatics
Accurate prediction of binding poses is crucial to structure-based drug design. We employ two powerful artificial intelligence (AI) approaches, data-mining and machine-learning, to design artificial neural network (ANN) based pose-scoring function. I...

Base-resolution prediction of transcription factor binding signals by a deep learning framework.

PLoS computational biology
Transcription factors (TFs) play an important role in regulating gene expression, thus the identification of the sites bound by them has become a fundamental step for molecular and cellular biology. In this paper, we developed a deep learning framewo...

Inferring RNA-binding protein target preferences using adversarial domain adaptation.

PLoS computational biology
Precise identification of target sites of RNA-binding proteins (RBP) is important to understand their biochemical and cellular functions. A large amount of experimental data is generated by in vivo and in vitro approaches. The binding preferences det...

Using Steady-State Kinetics to Quantitate Substrate Selectivity and Specificity: A Case Study with Two Human Transaminases.

Molecules (Basel, Switzerland)
We examined the ability of two human cytosolic transaminases, aspartate aminotransferase (GOT1) and alanine aminotransferase (GPT), to transform their preferred substrates whilst discriminating against similar metabolites. This offers an opportunity ...

Machine Learning Approaches for Metalloproteins.

Molecules (Basel, Switzerland)
Metalloproteins are a family of proteins characterized by metal ion binding, whereby the presence of these ions confers key catalytic and ligand-binding properties. Due to their ubiquity among biological systems, researchers have made immense efforts...

Positional SHAP (PoSHAP) for Interpretation of machine learning models trained from biological sequences.

PLoS computational biology
Machine learning with multi-layered artificial neural networks, also known as "deep learning," is effective for making biological predictions. However, model interpretation is challenging, especially for sequential input data used with recurrent neur...

Predicting Ca and Mg ligand binding sites by deep neural network algorithm.

BMC bioinformatics
BACKGROUND: Alkaline earth metal ions are important protein binding ligands in human body, and it is of great significance to predict their binding residues.