Protein science : a publication of the Protein Society
Sep 1, 2025
Despite the vast number of enzymatic kinetic measurements reported across decades of biochemical literature, the majority of relational enzyme kinetic data-linking amino acid sequence, substrate identity, kinetic parameters, and assay conditions-rema...
Biotechnology advances
Sep 1, 2025
Catalytic promiscuity in enzymes refers to their ability to catalyze multiple chemically distinct reactions in addition to their native activity. The increasing discovery of additional enzymes exhibiting catalytic promiscuity has underscored the sign...
Biotechnology advances
Sep 1, 2025
Enzymes are indispensable for biological processes and diverse applications across industries. While top-down modification strategies, such as directed evolution, have achieved remarkable success in optimizing existing enzymes, bottom-up de novo enzy...
Materials horizons
Aug 11, 2025
Nanozymes, an emerging class of artificial enzymes, have attracted increasing attention for their potential in environmental monitoring, industrial catalysis, food safety, and biomedicine. To date, more than 1500 nanomaterials have been identified wi...
Nucleic acids research
Jul 7, 2025
DeepMolecules is an easily accessible web server for predicting protein-small molecule interactions. It integrates four state-of-the-art models: ESP and SPOT for identifying substrates of enzymes and transporters, respectively, TurNuP for predicting ...
Computers in biology and medicine
Jul 1, 2025
Accurate prediction of the turnover number (k), which quantifies the maximum rate of substrate conversion at an enzyme's active site, is essential for assessing catalytic efficiency and understanding biochemical reaction mechanisms. Traditional wet-l...
Journal of agricultural and food chemistry
May 28, 2025
Mycotoxins are toxic fungal metabolites that pose significant health risks. Enzyme biotransformation is a promising option for detoxifying mycotoxins and for elucidating their intracellular metabolism. However, few mycotoxin-biotransformation enzymes...
Molecular cell
Apr 3, 2025
Enzyme design has struggled to emulate the complexity and catalytic proficiency of natural enzymes. Lauko et al. show that with the help of deep learning, the design of serine hydrolases that rival nature's ingenuity is possible.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Mar 25, 2025
Natural components serve the survival instincts of cells that are obtained through long-term evolution, while they often fail to meet the demands of engineered cells for efficiently performing biological functions in special industrial environments. ...
Briefings in bioinformatics
Mar 4, 2025
The prediction of enzyme kinetic parameters is crucial for screening enzymes with high catalytic efficiency and desired characteristics to catalyze natural or non-natural reactions. Data-driven machine learning models have been explored to reduce exp...