AIMC Topic: Biocatalysis

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Renewable Phytase Biocatalyst to Transform Biorefinery Waste Streams into Phosphorus Resources.

Environmental science & technology
Phosphorus (P) recovery is crucial for sustaining the global food supply and preventing freshwater pollution. Biorefinery waste streams have emerged as promising, yet underexplored sources for P recovery. Here, we presented a renewable and robust cel...

Characterization of a Novel Mutansucrase (MUT-I) from G29: Enzymatic Properties and Product Analysis.

Journal of agricultural and food chemistry
Glucansucrases are extracellular enzymes capable of synthesizing diverse α-glucan polymers and oligosaccharides, including the industrially relevant mutan. The I-encoded mutansucrase (MUT-I) from G29 was biochemically characterized as a robust bioca...

Glycolysis-compatible urethanases for polyurethane recycling.

Science (New York, N.Y.)
Recycling thermoset polyurethanes is hindered by their cross-linked structures and chemically stable urethane bonds. Although chemo-enzymatic approaches offer promise, known urethanases remain inefficient under industrial glycolysis conditions. Here,...

Artificial Intelligence-Driven Design of Robust Enzymes to Enhance Their Performance.

ACS synthetic biology
The booming artificial intelligence (AI) technology provides an opportunity to precisely carry out design of enzymes and create new biocatalysts with significantly enhanced performance. In the past decade, successful enzyme design cases, although t...

TCNeKP: A Novel Deep Learning Architecture for Enzyme Catalytic Activity Prediction.

Journal of chemical information and modeling
Accurate prediction of enzyme kinetic parameters ( and ) is crucial for enzyme rational design and engineering research. Based on a heterogeneous data set encompassing 17,893 and 24,585 records across 8911 enzyme sequences from 7 EC classes and 502...

Thermodynamic Microenvironment Engineering in Mesoporous Nanoreactors to Enhance Biocatalysis for AI-Empowered Ultrasensitive Pathogen Detection.

Analytical chemistry
Harmonizing enzyme-support microenvironments to govern thermodynamic interaction landscapes presents a critical yet underexplored frontier in nanobiocatalysis for pathogen detection. Herein, we architecturally engineer mesoporous resorcinol formaldeh...

Computer-Aided Techniques in the Engineering of Enzyme Binding Pockets: New Perspectives and Frontiers.

Journal of agricultural and food chemistry
Enzymes, recognized for their remarkable catalytic efficiency, play a crucial role in a myriad of biochemical reactions. However, the catalytic performance of natural enzymes frequently does not meet the demands of specific applications. To address t...

ESM-Ezy: a deep learning strategy for the mining of novel multicopper oxidases with superior properties.

Nature communications
The UniProt database is a valuable resource for biocatalyst discovery, yet predicting enzymatic functions remains challenging, especially for low-similarity sequences. Identifying superior enzymes with enhanced catalytic properties is even harder. To...

Enhancing the synthesis efficiency of galacto-oligosaccharides of a β-galactosidase from Paenibacillus barengoltzii by engineering the active and distal sites.

Food chemistry
Previously, a glycoside hydrolase (GH) family 2 β-galactosidase (PbBGal2A) from Paenibacillus barengoltzii is characterized for its high transglycosylation capability. Here, the cryo-electron microscopy (cryo-EM) structure of PbBGal2A was determined,...

Graphene-based FETs for advanced biocatalytic profiling: investigating heme peroxidase activity with machine learning insights.

Mikrochimica acta
Graphene-based field-effect transistors (GFETs) are rapidly gaining recognition as powerful tools for biochemical analysis due to their exceptional sensitivity and specificity. In this study, we utilize a GFET system to explore the peroxidase-based b...