AIMC Topic: Mutagenesis, Site-Directed

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Development and characterization of an α-l-rhamnosidase mutant with improved thermostability and a higher efficiency for debittering orange juice.

Food chemistry Nov 16, 2017
The glycoside hydrolase, α-l-rhamnosidase, could remove the bitter taste of naringin from citrus juices. However, most α-l-rhamnosidases are easily deactivated at high temperatures, limiting the practice in debittering citrus juices. The V529A mutant...
Temperature Fruit and Vegetable Juices Taste Aspergillus niger Glycoside Hydrolases Citrus sinensis Directed Molecular Evolution Mutagenesis, Site-Directed Protein Engineering
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modeling of the cryptic E2∼ubiquitin-binding site of E6-associated protein (E6AP)/UBE3A reveals the mechanism of polyubiquitin chain assembly.

The Journal of biological chemistry Sep 18, 2017
To understand the mechanism for assembly of Lys-linked polyubiquitin degradation signals, we previously demonstrated that the E6AP/UBE3A ligase harbors two functionally distinct E2∼ubiquitin-binding sites: a high-affinity Site 1 required for E6AP Cys...
Humans Animals Molecular Docking Simulation Cattle Computational Biology Models, Molecular Iodine Radioisotopes Expert Systems Biocatalysis Protein Conformation Cysteine Ubiquitin-Protein Ligases Amino Acid Substitution Recombinant Fusion Proteins Binding Sites Mutagenesis, Site-Directed Ubiquitin Ubiquitination Protein Multimerization Isoenzymes Point Mutation Ubiquitin-Conjugating Enzymes
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