Weissella cibaria EIR/P2-derived exopolysaccharide: A novel alternative to conventional biomaterials targeting periodontal regeneration.

Journal: International journal of biological macromolecules
PMID: 33736289

Abstract

Healing and regeneration of periodontium are considered as a complex physiological process. Therefore, treatments need to be addressed with highly effective components modulating the multiple pathways. In this study, exopolysaccharide (EPS) produced by Weissella cibaria EIR/P2, was partially purified from the culture supernatant and subjected to characterization within the aim of evaluating its potential for periodontal regeneration. High-Performance Liquid Chromatography analysis revealed a single-peak corresponding to the glucose which identified the EPS as dextran. Fourier transform-infrared spectra were also displayed characteristic peaks for polysaccharides. According to the results of gel permeation/size exclusion-chromatography, the molecular mass was determined to be 8 × 10 Da. To clarify its anti-bacterial activity on Streptococcus mutans, effects on viability and biofilm formation was evaluated. At 50 mg/mL, dextran exhibited a bactericidal effect with 70% inhibition on biofilm formation. Besides, dose-dependent antioxidant effects were also detected. The efficacy of dextran in enhancing the viability of human periodontal ligament fibroblast cells (hPDLFCs) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay, and an increase was observed in the viability of hPDLFCs. In conclusion, dextran derived from W. cibaria can be potentially used as a multi-functional bioactive polymer in the design of new therapeutic strategies to promote healing and regeneration of periodontium.

Authors

  • Hazal Kibar
    Pharmabiotic Technologies Research Laboratory, Department of Biology, Faculty of Science, Ankara University, 06100 Ankara, Turkey; Regenerative Biomaterials Laboratory, Department of Bioengineering, Faculty of Engineering, Canakkale Onsekiz Mart University, 17100 Canakkale, Turkey.
  • Yavuz Emre Arslan
    Regenerative Biomaterials Laboratory, Department of Bioengineering, Faculty of Engineering, Canakkale Onsekiz Mart University, 17100 Canakkale, Turkey.
  • Ahmet Ceylan
    Department of Histology and Embryology, Faculty of Veterinary Medicine, Ankara University, 06110 Ankara, Turkey.
  • Başar Karaca
    Microbiology Research Laboratory, Department of Biology, Faculty of Science, Ankara University, 06100 Ankara, Turkey.
  • Ozan Haliscelik
    Pharmabiotic Technologies Research Laboratory, Department of Biology, Faculty of Science, Ankara University, 06100 Ankara, Turkey.
  • Fadime Kiran
    Pharmabiotic Technologies Research Laboratory, Department of Biology, Faculty of Science, Ankara University, 06100 Ankara, Turkey. Electronic address: fkiran@science.ankara.edu.tr.

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