Single molecule localisation microscopy (SMLM) can provide two-dimensional super-resolved image data from conventional fluorescence microscopes, while three dimensional (3D) SMLM usually involves a modification of the microscope, for example, to engi...
Diseases like bronchopulmonary dysplasia (BPD) affect the development of the pulmonary vasculature, including the alveolar capillary network (ACN). Since pulmonary development is highly dependent on angiogenesis and microvascular maturation, ACN inve...
The types and quantities of microorganisms in activated sludge are directly related to the stability and efficiency of sewage treatment systems. This paper proposes a sludge microorganism detection method based on microscopic phase contrast image opt...
Micropatterning is reliable method for quantifying pluripotency of human-induced pluripotent stem cells (hiPSCs) that differentiate to form a spatial pattern of sorted, ordered and nonoverlapped three germ layers on the micropattern. In this study, w...
Optical mesoscale imaging is a rapidly developing field that allows the visualisation of larger samples than is possible with standard light microscopy, and fills a gap between cell and organism resolution. It spans from advanced fluorescence imaging...
In this paper, we present a methodology for measuring the reaction degree of ground granulated blast furnace slag (GGBFS) in alkali-activated cements using neural network based image analysis. The new methodology consists of an image analysis routine...
Optical microscopes have long been essential for many scientific disciplines. However, the resolution and contrast of such microscopic images are dramatically affected by aberrations. In this study, compacted with adaptive optics, we propose a machin...
We presenta robust, long-range optical autofocus system for microscopy utilizing machine learning. This can be useful for experiments with long image data acquisition times that may be impacted by defocusing resulting from drift of components, for ex...
Identifying nuclei is a standard first step when analysing cells in microscopy images. The traditional approach relies on signal from a DNA stain, or fluorescent transgene expression localised to the nucleus. However, imaging techniques that do not u...
Through-focus scanning optical microscopy (TSOM) is an economical, non-contact and nondestructive method for rapid measurement of three-dimensional nanostructures. There are two methods using TSOM image to measure the dimensions of one sample, includ...