AIMC Topic: Single Molecule Imaging

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Deep learning-assisted analysis of single-particle tracking for automated correlation between diffusion and function.

Nature methods May 8, 2025
Subcellular diffusion in living systems reflects cellular processes and interactions. Recent advances in optical microscopy allow the tracking of this nanoscale diffusion of individual objects with unprecedented precision. However, the agnostic and a...
Endosomes Deep Learning Image Processing, Computer-Assisted Diffusion Single Molecule Imaging Humans Software
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Single Molecule Localization Super-resolution Dataset for Deep Learning with Paired Low-resolution Images.

Scientific data Apr 23, 2025
Deep learning super-resolution microscopy has advanced rapidly in recent years. Super-resolution images acquired by single molecule localization microscopy (SMLM) are ideal sources for high-quality datasets. However, the scarcity of public datasets l...
Image Processing, Computer-Assisted Microscopy, Fluorescence Deep Learning Single Molecule Imaging Endoplasmic Reticulum
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A review of denoising methods in single-particle cryo-EM.

Micron (Oxford, England : 1993) Mar 29, 2025
Cryo-EM has become a vital technique for resolving macromolecular structures at near-atomic resolution, enabling the visualization of proteins and large molecular complexes. However, the images are often accompanied by extremely low SNR, which poses ...
Imaging, Three-Dimensional Image Processing, Computer-Assisted Deep Learning Signal-To-Noise Ratio Proteins Single Molecule Imaging Cryoelectron Microscopy Macromolecular Substances
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Deep Learning and Single-Molecule Localization Microscopy Reveal Nanoscopic Dynamics of DNA Entanglement Loci.

ACS nano Feb 4, 2025
Understanding molecular dynamics at the nanoscale remains challenging due to limitations in the temporal resolution of current imaging techniques. Deep learning integrated with Single-Molecule Localization Microscopy (SMLM) offers opportunities to pr...
Kinetics Nanotechnology Fluorescent Dyes Microscopy, Fluorescence Single Molecule Imaging Deep Learning DNA
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Pretrained Deep Neural Network Kin-SiM for Single-Molecule FRET Trace Idealization.

The journal of physical chemistry. B Jan 14, 2025
Single-molecule fluorescence resonance energy transfer (smFRET) has emerged as a pivotal technique for probing biomolecular dynamics over time at nanometer scales. Quantitative analyses of smFRET time traces remain challenging due to confounding fact...
Fluorescence Resonance Energy Transfer Single Molecule Imaging Kinetics Markov Chains Neural Networks, Computer Deep Learning
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Trajectory Analysis in Single-Particle Tracking: From Mean Squared Displacement to Machine Learning Approaches.

International journal of molecular sciences Aug 8, 2024
Single-particle tracking is a powerful technique to investigate the motion of molecules or particles. Here, we review the methods for analyzing the reconstructed trajectories, a fundamental step for deciphering the underlying mechanisms driving the m...
Software Algorithms Markov Chains Single Molecule Imaging Motion Machine Learning
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Deep learning-based spectroscopic single-molecule localization microscopy.

Journal of biomedical optics May 24, 2024
SIGNIFICANCE: Spectroscopic single-molecule localization microscopy (sSMLM) takes advantage of nanoscopy and spectroscopy, enabling sub-10 nm resolution as well as simultaneous multicolor imaging of multi-labeled samples. Reconstruction of raw sSMLM ...
Image Processing, Computer-Assisted Microscopy, Fluorescence Chlorocebus aethiops DNA, Single-Stranded Animals Deep Learning Humans Algorithms Histones Single Molecule Imaging COS Cells
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Deep Learning-Assisted Automated Multidimensional Single Particle Tracking in Living Cells.

Nano letters Feb 28, 2024
The translational and rotational dynamics of anisotropic optical nanoprobes revealed in single particle tracking (SPT) experiments offer molecular-level information about cellular activities. Here, we report an automated high-speed multidimensional S...
Deep Learning Biological Transport Single Molecule Imaging Gold Metal Nanoparticles
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Content-aware frame interpolation (CAFI): deep learning-based temporal super-resolution for fast bioimaging.

Nature methods Jan 18, 2024
The development of high-resolution microscopes has made it possible to investigate cellular processes in 3D and over time. However, observing fast cellular dynamics remains challenging because of photobleaching and phototoxicity. Here we report the i...
Single Molecule Imaging Motion Deep Learning Microscopy
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Deep-LASI: deep-learning assisted, single-molecule imaging analysis of multi-color DNA origami structures.

Nature communications Oct 17, 2023
Single-molecule experiments have changed the way we explore the physical world, yet data analysis remains time-consuming and prone to human bias. Here, we introduce Deep-LASI (Deep-Learning Assisted Single-molecule Imaging analysis), a software suite...
Fluorescence Resonance Energy Transfer DNA Humans Protein Conformation Single Molecule Imaging Microscopy Deep Learning
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