Proceedings of the National Academy of Sciences of the United States of America
Nov 24, 2025
Cryoelectron tomography (cryo-ET) enables three-dimensional visualization of molecular structures within tissue and intact cells, providing a powerful tool for studying the spatial organization of biological components at nanometer resolution. Realiz...
Proceedings of the National Academy of Sciences of the United States of America
Nov 3, 2025
Self-assembling protein nanoparticles are being increasingly utilized in the design of next-generation vaccines due to their ability to induce antibody responses of superior magnitude, breadth, and durability. Computational protein design offers a ro...
Cryo-EM and X-ray crystallography provide crucial experimental data for obtaining atomic-detail models of biomacromolecules. Refining these models relies on library-based stereochemical data, which, in addition to being limited to known chemical enti...
Cryo-electron tomography enables the visualization of macromolecular complexes within native cellular environments but is limited by incomplete angular sampling and the maximal electron dose that biological specimens can be exposed to. Here, we devel...
Cryo-electron tomography (cryo-ET) enables three-dimensional visualization of biomolecules and cellular components in their near-native state. A key challenge in cryo-ET data analysis is particle picking, often performed by template matching, which r...
The transfection potency and biological fate of gene-loaded lipid nanoparticles (LNPs) are often determined by their morphological and physicochemical properties. Cryogenic-electron microscopy (cryo-EM) remains the most effective tool to analyze LNP ...
CRISPR-Cas systems revolutionize life science. Metagenomes contain millions of unknown Cas proteins. Traditional mining relies on protein sequence alignments. In this work, we employ an evolutionary scale language model (ESM) to learn the information...
Iron is an essential nutrient for most bacteria and is often growth-limiting during infection, due to the host sequestering free iron as part of the innate immune response. To obtain the iron required for growth, many bacterial pathogens encode trans...
At sufficiently high resolution, x-ray crystallography and cryogenic electron microscopy are capable of resolving small spherical map features corresponding to either water or ions. Correct classification of these sites provides crucial insight for u...
Proceedings of the National Academy of Sciences of the United States of America
Jun 4, 2025
Characterizing the conformational ensemble of biomolecular systems is key to understand their functions. Cryoelectron microscopy (cryo-EM) captures two-dimensional snapshots of biomolecular ensembles, giving in principle access to thermodynamics. How...
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