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Genotyping Techniques

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High throughput preparation of fly genomic DNA in 96-well format using a paint-shaker.

Fly
Sample homogenization is an essential step for genomic DNA extraction, with multiple downstream applications in Molecular Biology. Genotyping hundreds or thousands of samples requires an automation of this homogenization step, and high throughput hom...

Automated Classification and Cluster Visualization of Genotypes Derived from High Resolution Melt Curves.

PloS one
INTRODUCTION: High Resolution Melting (HRM) following PCR has been used to identify DNA genotypes. Fluorescent dyes bounded to double strand DNA lose their fluorescence with increasing temperature, yielding different signatures for different genotype...

Characterizing root response phenotypes by neural network analysis.

Journal of experimental botany
Roots play an immediate role as the interface for water acquisition. To improve sustainability in low-water environments, breeders of major crops must therefore pay closer attention to advantageous root phenotypes; however, the complexity of root arc...

A Framework for Identifying Genotypic Information from Clinical Records: Exploiting Integrated Ontology Structures to Transfer Annotations between ICD Codes and Gene Ontologies.

IEEE/ACM transactions on computational biology and bioinformatics
Although some methods are proposed for automatic ontology generation, none of them address the issue of integrating large-scale heterogeneous biomedical ontologies. We propose a novel approach for integrating various types of ontologies efficiently a...

Assessing and comparison of different machine learning methods in parent-offspring trios for genotype imputation.

Journal of theoretical biology
Genotype imputation is an important tool for prediction of unknown genotypes for both unrelated individuals and parent-offspring trios. Several imputation methods are available and can either employ universal machine learning methods, or deploy algor...