AIMC Topic: Photobleaching

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Automated Stoichiometry Analysis of Single-Molecule Fluorescence Imaging Traces via Deep Learning.

Journal of the American Chemical Society Apr 18, 2019
The stoichiometry of protein complexes is precisely regulated in cells and is fundamental to protein function. Singe-molecule fluorescence imaging based photobleaching event counting is a new approach for protein stoichiometry determination under phy...
Humans Algorithms Fluorescence Deep Learning Fluorescent Dyes HeLa Cells DNA, Single-Stranded Carbocyanines ErbB Receptors Single Molecule Imaging Protein Structure, Quaternary Photobleaching
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Reduction of photobleaching effects in photoacoustic imaging using noise agnostic, platform-flexible deep-learning methods.

Journal of biomedical optics Dec 1, 2025
SIGNIFICANCE: Molecular photoacoustic (PA) imaging with exogenous dyes faces a significant challenge due to the photobleaching of the dye that can compromise tissue visualization, particularly in 3D imaging. Addressing this limitation can revolutioni...
Humans Animals Deep Learning Imaging, Three-Dimensional Image Processing, Computer-Assisted Mice Signal-To-Noise Ratio Photoacoustic Techniques Photobleaching
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Enhancing fluorescent protein photostability through robot-assisted photobleaching.

Integrative biology : quantitative biosciences from nano to macro Jul 16, 2018
Improving fluorescent proteins through the use of directed evolution requires robust techniques for screening large libraries of genetic variants. Here we describe an effective and relatively low-cost system for screening libraries of fluorescent pro...
Humans Escherichia coli Robotics Bacterial Proteins Mutation Models, Biological Light HeLa Cells Plasmids Crystallography, X-Ray Luminescent Proteins Photobleaching Photochemistry
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