Optimal structural characteristics of osteoinductivity in bioceramics derived from a novel high-throughput screening plus machine learning approach.

Journal: Biomaterials
PMID: 40262463

Abstract

Osteoinduction is an important feature of the next generation of bone repair materials. But the key structural factors and parameters of osteoinductive scaffolds are not yet clarified. This study leverages the efficiency of high-throughput screening in identifying key structural factors, performs screening of 24 different porous structures using 3D printed calcium phosphate (CaP) ceramic scaffolds. Based on in vitro and in vivo evaluations, along with machine learning and nonlinear fitting, it explores the complex relationship between osteoinductive properties and scaffold configurations. Results indicate that bone regenerative ability is largely affected by porosity and specific surface area (SSA), while pore geometry has a negligible effect. The optimal structural parameters were identified as a porous structure with SSA of 10.49-10.69 mm mm and permeability of 3.74 × 10 m, which enhances osteoinductivity and scaffold properties, corresponding to approximately 65 %-70 % porosity. Moreover, nonlinear fitting reveals specific correlations among SSA, permeability and osteogenic gene expressions. We established a data-driven high-throughput screening methodology and proposed a parametric benchmark for osteoinductive structures, providing critical insights for the design of future osteoinductive scaffolds.

Authors

  • Yunyi Liu
    National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China; College of Biomedical Engineering, Sichuan University, Chengdu, 610064, China.
  • Quanle Cao
    National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China; College of Biomedical Engineering, Sichuan University, Chengdu, 610064, China.
  • Shengyi Yong
    National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China; College of Biomedical Engineering, Sichuan University, Chengdu, 610064, China.
  • Jing Wang
    Endoscopy Center, Peking University Cancer Hospital and Institute, Beijing, China.
  • Xuening Chen
    Provincial Engineering Research Center for Biomaterials Genome of Sichuan & Research Center for Material Genome Engineering, Sichuan University, Chengdu, 610064, China; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China; College of Biomedical Engineering, Sichuan University, Chengdu, 610064, China.
  • Yumei Xiao
    Provincial Engineering Research Center for Biomaterials Genome of Sichuan & Research Center for Material Genome Engineering, Sichuan University, Chengdu, 610064, China; National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China; College of Biomedical Engineering, Sichuan University, Chengdu, 610064, China.
  • Jiangli Lin
    Department of Biomedical Engineering, College of Materials Science and Engineering, Sichuan University, Chengdu, China.
  • Mingli Yang
    College of Biomedical Engineering, Sichuan University, Chengdu 610065, China; Provincial Engineering Research Center for Biomaterials Genome of Sichuan, Sichuan University, Chengdu 610065, China; National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu 610065, China.
  • Kefeng Wang
    College of Biomedical Engineering, Sichuan University, Chengdu 610065, China; Provincial Engineering Research Center for Biomaterials Genome of Sichuan, Sichuan University, Chengdu 610065, China; National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu 610065, China.
  • Xiangfeng Li
    College of Biomedical Engineering, Sichuan University, Chengdu 610065, China; Provincial Engineering Research Center for Biomaterials Genome of Sichuan, Sichuan University, Chengdu 610065, China; National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu 610065, China. Electronic address: hkdlixiangfeng@163.com.
  • Xiangdong Zhu
    College of Biomedical Engineering, Sichuan University, Chengdu 610065, China; Provincial Engineering Research Center for Biomaterials Genome of Sichuan, Sichuan University, Chengdu 610065, China; National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu 610065, China. Electronic address: zhu_xd1973@scu.edu.cn.
  • Xingdong Zhang
    College of Biomedical Engineering, Sichuan University, Chengdu 610065, China; Provincial Engineering Research Center for Biomaterials Genome of Sichuan, Sichuan University, Chengdu 610065, China; National Engineering Research Centre for Biomaterials, Sichuan University, Chengdu 610065, China.

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