Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
This chapter proposes a prototype-based classification approach for analyzing DNA barcodes that uses a spectral representation of DNA sequences and a non-gradient neural network. Biological sequences can be viewed as data components with higher non-f...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
Liquid chromatography-coupled mass spectrometry (LC-MS/MS) is the primary method to obtain direct evidence for the presentation of disease- or patient-specific human leukocyte antigen (HLA). However, compared to the analysis of tryptic peptides in pr...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
Peptide therapeutics is gaining momentum. Advances in the field of peptidomics have enabled researchers to harvest vital information from various organisms and tissue types concerning peptide existence, expression and function. The development of mas...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
Flow cytometry (FC) is routinely used for hematological disease diagnosis and monitoring. Advancement in this technology allows us to measure an increasing number of markers simultaneously, generating complex high-dimensional datasets. However, curre...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
Protein-protein interactions (PPIs) are known to be involved in most cellular functions, and a detailed knowledge of such interactions is essential for studying their role in normal and pathological conditions. Significant progress is being made in t...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
Antigen-specific B-cell ELISPOT and multicolor FluoroSpot assays, in which the membrane-bound antigen itself serves as the capture reagent for the antibodies that B cells secrete, inherently result in a broad range of spot sizes and intensities. The ...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
Knowledge regarding cancer stem cell (CSC) morphology is limited, and more extensive studies are therefore required. Image recognition technologies using artificial intelligence (AI) require no previous expertise in image annotation. Herein, we descr...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
The identification of essential genes is a key challenge in systems and synthetic biology, particularly for engineering metabolic pathways that convert feedstocks into valuable products. Assessment of gene essentiality at a genome scale requires larg...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
We briefly present machine learning approaches for designing better biological experiments. These approaches build on machine learning predictors and provide additional tools to guide scientific discovery. There are two different kinds of objectives ...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2024
Mathematical modeling plays a vital role in mammalian synthetic biology by providing a framework to design and optimize design circuits and engineered bioprocesses, predict their behavior, and guide experimental design. Here, we review recent models ...