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Cryopreservation

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Experience With Hypothermic Machine Perfusion in Expanded Criteria Donors: Functional Outcomes.

Transplantation proceedings
UNLABELLED: Hypothermic machine perfusion (HMP) decreases delayed graft function (DGF) and improves 1-year graft survival in expanded criteria donors (ECDs). Time of HMP could be associated with incidence of DGF.

Effect of Dilution on Cryosurvival of Low Sperm Doses: A Review.

Cryo letters
  BACKGROUND: Sperm concentration needed for artificial insemination (AI) to obtain reasonable fertility, taking into consideration genetic value of bull and numerous others components is one of the essential constituents for successful breeding prog...

Canonical correlation analysis to identify the semen characteristics used to forecast the freeze survival of Curimba (Prochilodus lineatus) spermatozoa.

Cryo letters
UNLABELLED:   OBJECTIVE: To identify which sperm characteristics were able to predict more accurately the quality of curimba (Prochilodus lineatus) semen upon freezing using canonical correlation analysis.

Dietary polyunsaturated fatty acid supplementation of young post-pubertal dairy bulls alters the fatty acid composition of seminal plasma and spermatozoa but has no effect on semen volume or sperm quality.

Theriogenology
The aim of this study was to examine the effects of dietary supplementation with rumen protected n-6 or n-3 polyunsaturated fatty acids (PUFA) on the quantity and quality of semen from young post-pubertal dairy bulls. Pubertal Holstein-Friesian (n = ...

Sperm quality and its freezing ability throughout the spawning season in Prochilodus lineatus and Brycon orbignyanus.

Theriogenology
The aim of this study was to determine fresh and frozen sperm quality evaluated over two spawning seasons (2013-2014; 2014-2015) in Prochilodus lineatus and Brycon orbignyanus. The spawning seasons were divided into two sampling periods: November to ...

Toxoplasma gondii transmission by artificial insemination in sheep with experimentally contaminated frozen semen.

Theriogenology
Toxoplasma gondii is a parasite considered one of the major causes of reproductive problems in sheep. Furthermore, the presence of the agent in ram semen urges the possibility of sexual transmission in this species. The aim of this study was to evalu...

Controlled ice nucleation--Is it really needed for large-volume sperm cryopreservation?

Theriogenology
Controlled ice nucleation (CIN) is an integral stage of slow freezing process when relatively large volumes (usually 1 mL or larger) of biological samples in suspension are involved. Without it, a sample will supercool to way below its melting point ...

Convolutional neural networks for automated annotation of cellular cryo-electron tomograms.

Nature methods
Cellular electron cryotomography offers researchers the ability to observe macromolecules frozen in action in situ, but a primary challenge with this technique is identifying molecular components within the crowded cellular environment. We introduce ...

The application of convolution neural network based cell segmentation during cryopreservation.

Cryobiology
For most of the cells, water permeability and plasma membrane properties play a vital role in the optimal protocol for successful cryopreservation. Measuring the water permeability of cells during subzero temperature is essential. So far, there is no...