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High-Throughput Screening Assays

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Colony analysis and deep learning uncover 5-hydroxyindole as an inhibitor of gliding motility and iridescence in Cellulophaga lytica.

Microbiology (Reading, England)
Iridescence is an original type of colouration that is relatively widespread in nature but has been either incompletely described or entirely neglected in prokaryotes. Recently, we reported a brilliant 'pointillistic' iridescence in agar-grown colony...

Hit Dexter: A Machine-Learning Model for the Prediction of Frequent Hitters.

ChemMedChem
False-positive assay readouts caused by badly behaving compounds-frequent hitters, pan-assay interference compounds (PAINS), aggregators, and others-continue to pose a major challenge to experimental screening. There are only a few in silico methods ...

: High-Throughput Quantification of Fluorescent Synaptic Protein Puncta by Machine Learning.

eNeuro
Synapse formation analyses can be performed by imaging and quantifying fluorescent signals of synaptic markers. Traditionally, these analyses are done using simple or multiple thresholding and segmentation approaches or by labor-intensive manual anal...

Integrating DNA structure switch with branched hairpins for the detection of uracil-DNA glycosylase activity and inhibitor screening.

Talanta
The detection of uracil-DNA glycosylase (UDG) activity is pivotal for its biochemical studies and the development of drugs for UDG-related diseases. Here, we explored an integrated DNA structure switch for high sensitive detection of UDG activity. Th...

Feature selection method based on support vector machine and shape analysis for high-throughput medical data.

Computers in biology and medicine
Proteomics data analysis based on the mass-spectrometry technique can provide a powerful tool for early diagnosis of tumors and other diseases. It can be used for exploring the features that reflect the difference between samples from high-throughput...

Machine Learning of Human Pluripotent Stem Cell-Derived Engineered Cardiac Tissue Contractility for Automated Drug Classification.

Stem cell reports
Accurately predicting cardioactive effects of new molecular entities for therapeutics remains a daunting challenge. Immense research effort has been focused toward creating new screening platforms that utilize human pluripotent stem cell (hPSC)-deriv...

Digging deep into Golgi phenotypic diversity with unsupervised machine learning.

Molecular biology of the cell
The synthesis of glycans and the sorting of proteins are critical functions of the Golgi apparatus and depend on its highly complex and compartmentalized architecture. High-content image analysis coupled to RNA interference screening offers opportuni...

Label-free detection of cellular drug responses by high-throughput bright-field imaging and machine learning.

Scientific reports
In the last decade, high-content screening based on multivariate single-cell imaging has been proven effective in drug discovery to evaluate drug-induced phenotypic variations. Unfortunately, this method inherently requires fluorescent labeling which...

Classification and analysis of a large collection of in vivo bioassay descriptions.

PLoS computational biology
Testing potential drug treatments in animal disease models is a decisive step of all preclinical drug discovery programs. Yet, despite the importance of such experiments for translational medicine, there have been relatively few efforts to comprehens...