AIMC Topic: Microscopy, Phase-Contrast

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Towards Fluorescent-Tag-Less Viral Titration: Automated Estimation of Cell-Size Distribution and Infection Level from Phase-Contrast Microscopy Using Deep Learning and Transfer Learning.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Automated detection of infected insect cells is one of the crucial tasks in the field of recombinant protein production and vaccine development. The major challenge lies in manual segmentation of cells and quantifying cell size distribution is tediou...

Rapid fiber-detection technique by artificial intelligence in phase-contrast microscope images of simulated atmospheric samples.

Annals of work exposures and health
Since the manufacture, import, and use of asbestos products have been completely abolished in Japan, the main cause of asbestos emissions into the atmosphere is the demolition and removal of buildings built with asbestos-containing materials. To dete...

AI-based Apoptosis Cell Classification Using Phase-contrast Images of K562 Cells.

Anticancer research
BACKGROUND/AIM: This study aimed to automate the classification of cells, particularly in identifying apoptosis, using artificial intelligence (AI) in conjunction with phase-contrast microscopy. The objective was to reduce reliance on manual observat...

Untrained deep learning-based differential phase-contrast microscopy.

Optics letters
Quantitative differential phase-contrast (DPC) microscopy produces phase images of transparent objects based on a number of intensity images. To reconstruct the phase, in DPC microscopy, a linearized model for weakly scattering objects is considered;...

High space-bandwidth in quantitative phase imaging using partially spatially coherent digital holographic microscopy and a deep neural network.

Optics express
Quantitative phase microscopy (QPM) is a label-free technique that enables monitoring of morphological changes at the subcellular level. The performance of the QPM system in terms of spatial sensitivity and resolution depends on the coherence propert...

GPU-based deep convolutional neural network for tomographic phase microscopy with ℓ1 fitting and regularization.

Journal of biomedical optics
Tomographic phase microscopy (TPM) is a unique imaging modality to measure the three-dimensional refractive index distribution of transparent and semitransparent samples. However, the requirement of the dense sampling in a large range of incident ang...

A multi-stage random forest classifier for phase contrast cell segmentation.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
We present a machine learning based approach to automatically detect and segment cells in phase contrast images. The proposed method consists of a multi-stage classification scheme based on random forest (RF) classifier. Both low level and mid level ...