Anticancer activity of biologically synthesized silver and gold nanoparticles on mouse myoblast cancer cells and their toxicity against embryonic zebrafish.

Journal: Materials science & engineering. C, Materials for biological applications
PMID: 28183660

Abstract

The aim of this study was to evaluate the anticancer activity of bioinspired silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) against mouse myoblast cancer cells (CC). Both AgNPs and AuNPs were biologically synthesized using Spinacia oleracea Linn., aqueous leaves extract. UV-Vis. spectrophotometer, high resolution-transmission electron microscopy (HR-TEM), field emission-scanning electron microscopy (FE-SEM) and X-ray diffraction (XRD) studies supported the successful synthesis of AgNPs and AuNPs. Both these NPs have shown cytotoxicity against CC cells even at very low concentration (5μg/mL). Acridine orange/Ethidium bromide (AO/EB) dual staining confirmed the apoptotic morphological features. The levels of caspase enzymes (caspase-3 and caspase-7) were significantly up-regulated in NPs treated myoblast cells than the plant extract. Furthermore, in zebrafish embryo toxicity study, AgNPs showed 100% mortality at 3μg/mL concentration while AuNPs exhibited the same at much higher concentration (300mg/mL). Taken together, these results provide a preliminary guidance for the development of biomaterials based drugs to fight against the fatal diseases for example cancer.

Authors

  • Rajan Ramachandran
    Centre for Advanced Studies in Botany, School of Life Sciences, University of Madras, Guindy Campus, Chennai 600 025, Tamil Nadu, India.
  • Chandran Krishnaraj
    Department of Food Science & Technology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, Republic of Korea; M/s. Eureka Forbes Ltd, R & D Centre, Kudlu, Bangalore, India.
  • Allur Subramaniyan Sivakumar
    Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Republic of Korea.
  • Palaniappan Prasannakumar
    Advanced Biomedical Imaging Center, Department of Electronic Engineering, Chonbuk National University, Jeonju 54896, Republic of Korea.
  • V K Abhay Kumar
    M/s. Eureka Forbes Ltd, R & D Centre, Kudlu, Bangalore, India.
  • Kwan Seob Shim
    Department of Animal Biotechnology, Chonbuk National University, Jeonju 54896, Republic of Korea.
  • Chul-Gyu Song
    Advanced Biomedical Imaging Center, Department of Electronic Engineering, Chonbuk National University, Jeonju 54896, Republic of Korea.
  • Soon-Il Yun
    Department of Food Science & Technology, College of Agriculture & Life Sciences, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address: siyun@jbnu.ac.kr.

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