AIMC Topic: Enzyme Stability

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ActiMut-XGB: Predicting thermodynamic stability of point mutations for CALB with protein language model.

International journal of biological macromolecules Jun 1, 2025
Predicting the functional impact of single-point mutations on protein residual activity, especially after high-temperature incubation, is critical in protein engineering. We present an innovative machine learning model based on eXtreme Gradient Boost...
Fungal Proteins Enzyme Stability Machine Learning Models, Molecular Thermodynamics Point Mutation Lipase Protein Engineering
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Multidimensional computational strategies enhance the thermostability of alpha-galactosidase.

International journal of biological macromolecules Jun 1, 2025
Alpha-Galactosidase has significant industrial application value in food processing, animal nutrition and medical applications. Microbial-derived α-galactosidases predominate industrial implementation due to high productivity, yet their inherent ther...
Temperature Aspergillus Enzyme Stability Protein Engineering Thermodynamics alpha-Galactosidase Kinetics Mutation Molecular Dynamics Simulation
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ThermoLink: Bridging disulfide bonds and enzyme thermostability through database construction and machine learning prediction.

Protein science : a publication of the Protein Society Sep 1, 2024
Disulfide bonds, covalently formed by sulfur atoms in cysteine residues, play a crucial role in protein folding and structure stability. Considering their significance, artificial disulfide bonds are often introduced to enhance protein thermostabilit...
Models, Molecular Enzyme Stability Machine Learning Databases, Protein Disulfides Protein Folding
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'Set it and forget it': automated lab uses AI and robotics to improve proteins.

Nature Jan 1, 2024
Temperature Robotics Enzyme Stability Enzymes Artificial Intelligence Automation, Laboratory
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[Characterization of β-1, 4-mannanase from Bacillus pumilus and heterologous expression in Lactobacillus casei].

Wei sheng wu xue bao = Acta microbiologica Sinica Dec 4, 2015
OBJECTIVE: Lactobacillus casei is widely used in food production and feed industry. The aim of this study was to construct the recombinant expression mannanase Lb. casei.
beta-Mannosidase Gene Expression Lacticaseibacillus casei Enzyme Stability Bacterial Proteins Kinetics Bacillus Cloning, Molecular
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[Preparation of baicalein using thermophilic and sugar-tolerant beta-glucosidase].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica Dec 1, 2015
The reaction conditions of baicalin hydrolyzed into baicalein by a kind of thermophilic and sugar-tolerant beta-glucosidase were studied in this paper. The beta-glucosidase could catalyze baicalin into baicalein well in the acetic acid-sodium acetate...
Hot Temperature beta-Glucosidase Flavanones Kinetics Hydrolysis Biocatalysis Flavonoids Glucose Enzyme Stability
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[Alkaline-adapted beta-mannanase of Bacillus pumilus: gene heterologous expression and enzyme characterization].

Wei sheng wu xue bao = Acta microbiologica Sinica Nov 4, 2015
OBJECTIVE: We expressed a novel alkaline-adapted beta-mannanase gene and characterized the enzyme for potential industrial applications.
Alkalies Escherichia coli Bacillus Phylogeny Gene Expression Molecular Sequence Data Bacterial Proteins Enzyme Stability Amino Acid Sequence Kinetics beta-Mannosidase Base Sequence Cloning, Molecular Sequence Alignment
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