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Nucleic Acids

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The sweet detection of rolling circle amplification: Glucose-based electrochemical genosensor for the detection of viral nucleic acid.

Biosensors & bioelectronics
Herein, an isothermal padlock probe-based assay for the simple and portable detection of pathogens coupled with a glucose oxidase (GOx)-based electrochemical readout is reported. Infectious diseases remain a constant threat on a global scale, as in r...

Representability of algebraic topology for biomolecules in machine learning based scoring and virtual screening.

PLoS computational biology
This work introduces a number of algebraic topology approaches, including multi-component persistent homology, multi-level persistent homology, and electrostatic persistence for the representation, characterization, and description of small molecules...

A novel strategy for retention prediction of nucleic acids with their sequence information in ion-pair reversed phase liquid chromatography.

Talanta
In this work, retention behaviors of oligonucleotides and double-stranded deoxyribonucleic acids (dsDNAs) have been investigated in ion-pair reversed-phase liquid chromatography (IP-RPLC). We demonstrated that classic linear solvent strength (LSS) mo...

Exploration of the nanomedicine-design space with high-throughput screening and machine learning.

Nature biomedical engineering
Only a tiny fraction of the nanomedicine-design space has been explored, owing to the structural complexity of nanomedicines and the lack of relevant high-throughput synthesis and analysis methods. Here, we report a methodology for determining struct...

Mechanical Gripper Design and Force Analysis of Microplates for Automated High-Throughput Nucleic Acid Detection System.

Journal of nanoscience and nanotechnology
In the automated high-throughput nucleic acid detection system, we need to grip and transfer consumables frequently when carrying out multichannel nucleic acid detection. In order to ensure the efficiency of experiments and solve problems of the defl...

CD-NuSS: A Web Server for the Automated Secondary Structural Characterization of the Nucleic Acids from Circular Dichroism Spectra Using Extreme Gradient Boosting Decision-Tree, Neural Network and Kohonen Algorithms.

Journal of molecular biology
Nucleic acids exhibit a repertoire of conformational preference depending on the sequence and environment. Circular dichroism (CD) is an essential and valuable tool for monitoring such secondary structural conformations of nucleic acids. Nonetheless,...