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Protein Transport

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Design of diverse, functional mitochondrial targeting sequences across eukaryotic organisms using variational autoencoder.

Nature communications
Mitochondria play a key role in energy production and metabolism, making them a promising target for metabolic engineering and disease treatment. However, despite the known influence of passenger proteins on localization efficiency, only a few protei...

MLCPP 2.0: An Updated Cell-penetrating Peptides and Their Uptake Efficiency Predictor.

Journal of molecular biology
Cell-penetrating peptides (CPPs) translocate into the cell as various biologically active conjugates and possess numerous biomedical applications. Several machine learning-based predictors have been proposed in the past, but they mostly focus on iden...

Multi-scale deep learning for the imbalanced multi-label protein subcellular localization prediction based on immunohistochemistry images.

Bioinformatics (Oxford, England)
MOTIVATION: The development of microscopic imaging techniques enables us to study protein subcellular locations from the tissue level down to the cell level, contributing to the rapid development of image-based protein subcellular location prediction...

Selective chemical probes can untangle the complexity of the plant cell endomembrane system.

Current opinion in plant biology
The endomembrane system is critical for plant growth and development and understanding its function and regulation is of great interest for plant biology research. Small-molecule targeting distinctive endomembrane components have proven powerful tool...

Self-supervised deep learning encodes high-resolution features of protein subcellular localization.

Nature methods
Explaining the diversity and complexity of protein localization is essential to fully understand cellular architecture. Here we present cytoself, a deep-learning approach for fully self-supervised protein localization profiling and clustering. Cytose...

GraphLoc: a graph neural network model for predicting protein subcellular localization from immunohistochemistry images.

Bioinformatics (Oxford, England)
MOTIVATION: Recognition of protein subcellular distribution patterns and identification of location biomarker proteins in cancer tissues are important for understanding protein functions and related diseases. Immunohistochemical (IHC) images enable v...

Digitally predicting protein localization and manipulating protein activity in fluorescence images using 4D reslicing GAN.

Bioinformatics (Oxford, England)
MOTIVATION: While multi-channel fluorescence microscopy is a vital imaging method in biological studies, the number of channels that can be imaged simultaneously is limited by technical and hardware limitations such as emission spectra cross-talk. On...

In Silico Screening and Optimization of Cell-Penetrating Peptides Using Deep Learning Methods.

Biomolecules
Cell-penetrating peptides (CPPs) have great potential to deliver bioactive agents into cells. Although there have been many recent advances in CPP-related research, it is still important to develop more efficient CPPs. The development of CPPs by in s...

Interpretable feature extraction and dimensionality reduction in ESM2 for protein localization prediction.

Briefings in bioinformatics
As the application of large language models (LLMs) has broadened into the realm of biological predictions, leveraging their capacity for self-supervised learning to create feature representations of amino acid sequences, these models have set a new b...

Deep generative model for protein subcellular localization prediction.

Briefings in bioinformatics
Protein sequence not only determines its structure but also provides important clues of its subcellular localization. Although a series of artificial intelligence models have been reported to predict protein subcellular localization, most of them pro...