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Cellulase

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[Gene optimization and efficient expression of Trichoderma reesei Cel5A in Pichia pastoris].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Deficient activity of endo-1,4-beta-glucanase II (Cel5A) secreted by Trichoderma reesei is one of the challenges involved in effective cellulase saccharification of cellulosic substrates. Therefore, we expressed Cel5A in Pichia pastoris by constructi...

Chromatographic Study of Azintamide in Bulk Powder and in Pharmaceutical Formulation in the Presence of Its Degradation Form.

Journal of AOAC International
Two specific, sensitive, and precise stability-indicating chromatographic methods were developed, optimized, and validated for the determination of Azintamide (AZ) in the presence of its degradation product. The first method was TLC combined with the...

Review of lignocellulolytic enzyme activity analyses and scale-down to microplate-based assays.

Talanta
With the increasing use of enzymes in environmental applications, there is a need for analytical methods adapted to large factorial experiments. Existing reference methods are chemical and labor intensive and unsuitable to analyze in parallel a large...

Prediction of sugar yields during hydrolysis of lignocellulosic biomass using artificial neural network modeling.

Bioresource technology
The present investigation was carried out to study application of ANN as a tool for predicting sugar yields of pretreated biomass during hydrolysis process at various time intervals. Since it is known that biomass loading and particle size influences...

Fuzzy logic feedback control for fed-batch enzymatic hydrolysis of lignocellulosic biomass.

Bioprocess and biosystems engineering
A fuzzy logic feedback control system was developed for process monitoring and feeding control in fed-batch enzymatic hydrolysis of a lignocellulosic biomass, dilute acid-pretreated corn stover. Digested glucose from hydrolysis reaction was assigned ...

Estimation of fungal biomass using multiphase artificial neural network based dynamic soft sensor.

Journal of microbiological methods
Interest in low cost cellulase production has become a major challenge in recent years for biorefineries. Fed-batch fermentation of Trichoderma strains for the production of low cost cellulase is carried out on complex media that has various soluble ...