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Chemistry Techniques, Analytical

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A 6 Second Analytical Method for Quantitation of Tacrolimus in Whole Blood by Use of Laser Diode Thermal Desorption Tandem Mass Spectrometry.

The journal of applied laboratory medicine
BACKGROUND: Therapeutic drug monitoring of immunosuppressive drugs is imperative for organ transplant recipients. High-performance LC-MS/MS is considered gold standard; however, immunoassays provide rapid turnaround time. New technology was developed...

Review of lignocellulolytic enzyme activity analyses and scale-down to microplate-based assays.

Talanta
With the increasing use of enzymes in environmental applications, there is a need for analytical methods adapted to large factorial experiments. Existing reference methods are chemical and labor intensive and unsuitable to analyze in parallel a large...

Machine learning for the meta-analyses of microbial pathogens' volatile signatures.

Scientific reports
Non-invasive and fast diagnostic tools based on volatolomics hold great promise in the control of infectious diseases. However, the tools to identify microbial volatile organic compounds (VOCs) discriminating between human pathogens are still missing...

Advances in industrial biopharmaceutical batch process monitoring: Machine-learning methods for small data problems.

Biotechnology and bioengineering
Biopharmaceutical manufacturing comprises of multiple distinct processing steps that require effective and efficient monitoring of many variables simultaneously in real-time. The state-of-the-art real-time multivariate statistical batch process monit...

Benchmarking machine learning methods for comprehensive chemical fingerprinting and pattern recognition.

Journal of chromatography. A
Machine learning (ML) has been used previously to recognize particular patterns of constituent compounds. Here, ML is used with comprehensive chemical fingerprints that capture the distribution of all constituent compounds to flexibly perform various...

Automated Analytical Measurement Processes Using a Dual-Arm Robotic System.

SLAS technology
The demand for automation in the analytical laboratory is high. In contrast to well-automated bioscreening and high-throughput and high-content screening processes, analytical measurement procedures are complex in their structure and changing frequen...

Automated online coupling of robot-assisted single drop microextraction and liquid chromatography.

Journal of chromatography. A
A high-throughput and innovative setup has been developed to automate the online integration of single drop microextraction (SDME), liquid chromatography (LC) and high-resolution mass spectrometry (QqToF). SDME and LC were online hyphenated for the f...

Determining gradient conditions for peptide purification in RPLC with machine-learning-based retention time predictions.

Journal of chromatography. A
A strategy for determining a suitable solvent gradient in silico in preparative peptide separations is presented. The strategy utilizes a machine-learning-based method, called ELUDE, for peptide retention time predictions based on the amino acid sequ...

Recent advances in robotic protein sample preparation for clinical analysis and other biomedical applications.

Clinica chimica acta; international journal of clinical chemistry
Discovery of new protein biomarker candidates has become a major research goal in the areas of clinical chemistry, analytical chemistry, and biomedicine. These important species constitute the molecular target when it comes to diagnosis, prognosis, a...

Hybrid Models for the simulation and prediction of chromatographic processes for protein capture.

Journal of chromatography. A
The biopharmaceutical industries are continuously faced with the pressure to reduce the development costs and accelerate development time scales. The traditional approach of heuristic-based or platform process-based optimization is soon getting obsol...