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Microscopy, Fluorescence

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Quantitative assessment of cancer cell morphology and motility using telecentric digital holographic microscopy and machine learning.

Cytometry. Part A : the journal of the International Society for Analytical Cytology
The noninvasive, fast acquisition of quantitative phase maps using digital holographic microscopy (DHM) allows tracking of rapid cellular motility on transparent substrates. On two-dimensional surfaces in vitro, MDA-MB-231 cancer cells assume several...

Controlled surface morphology and hydrophilicity of polycaprolactone toward human retinal pigment epithelium cells.

Materials science & engineering. C, Materials for biological applications
Applying scaffolds as a bed to enhance cell proliferation and even differentiation is one of the treatment of retina diseases such as age-related macular degeneration (AMD) which deteriorating photoreceptors and finally happening blindness. In this s...

Automated Classification of Circulating Tumor Cells and the Impact of Interobsever Variability on Classifier Training and Performance.

Journal of immunology research
Application of personalized medicine requires integration of different data to determine each patient's unique clinical constitution. The automated analysis of medical data is a growing field where different machine learning techniques are used to mi...

Deep Learning-Based Image Restoration and Super-Resolution for Fluorescence Microscopy: Overview and Resources.

Methods in molecular biology (Clifton, N.J.)
Fluorescence microscopy is a key method for the visualization of cellular, subcellular, and molecular live-cell dynamics, enabling access to novel insights into mechanisms of health and disease. However, effects like phototoxicity, the fugitive natur...

A framework of multi-view machine learning for biological spectral unmixing of fluorophores with overlapping excitation and emission spectra.

Briefings in bioinformatics
The accuracy of assigning fluorophore identity and abundance, known as spectral unmixing, in biological fluorescence microscopy images remains a significant challenge due to the substantial overlap in emission spectra among fluorophores. In tradition...

[Comparative analysis of two assaysin detection of sperm DNA fragmentation index, flow cytometry and AI-based fluorescence microscopy, based on AO staining: A multicentre study].

Zhonghua nan ke xue = National journal of andrology
OBJECTIVE: To study the correlation, consistency, and variations between two assays of DNA fragmentation index based on acridine orange (AO) staining via AI-based fluorescence microscopy(AI-DFI), and flow cytometry (FCM-DFI) across multiple centers.

Improving flat fluorescence microscopy in scattering tissue through deep learning strategies.

Optics express
Intravital microscopy in small animals growingly contributes to the visualization of short- and long-term mammalian biological processes. Miniaturized fluorescence microscopy has revolutionized the observation of live animals' neural circuits. The te...

Quantitative Analysis of Differentiation Activity for Mouse Embryonic Stem Cells by Deep Learning for Cell Center Detection using Three-Dimensional Confocal Fluorescence Microscopy Images.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Accurate single cell segmentation provides means to monitor the behavior of single cell within a population of cells. Time-lapse fluorescence images are used to reveal heterogeneous nature of single mouse embryonic stem cell (ESC) colony and monitor ...

Deep Learning Empowered Fresnel-based Lensless Fluorescence Microscopy.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
Miniaturized fluorescence microscopy has revolutionized the way neuroscientists study the brain in-vivo. Recent developments in computational lensless imaging promise a next generation of miniaturized microscopes in lensless fluorescence microscopy. ...