Cytometry. Part A : the journal of the International Society for Analytical Cytology
Jan 25, 2020
Optical imaging technology that has the advantages of high sensitivity and cost-effectiveness greatly promotes the progress of nondestructive single-cell studies. Complex cellular image analysis tasks such as three-dimensional reconstruction call for...
Development of label-free methods for accurate classification of cells with high throughput can yield powerful tools for biological research and clinical applications. We have developed a deep neural network of DINet for extracting features from cros...
International journal of molecular sciences
Dec 20, 2019
Advances in flow cytometry enable the acquisition of large and high-dimensional data sets per patient. Novel computational techniques allow the visualization of structures in these data and, finally, the identification of relevant subgroups. Correct ...
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Dec 6, 2019
Understanding the intricacies of particle-cell interactions is essential for many applications such as imaging, phototherapy, and drug/gene delivery, because it is the key to accurate control of the particle properties for the improvement of their th...
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Nov 5, 2019
High-throughput single-cell cytometry technologies have significantly improved our understanding of cellular phenotypes to support translational research and the clinical diagnosis of hematological and immunological diseases. However, subjective and ...
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Nov 5, 2019
Imaging flow cytometry (IFC) produces up to 12 spectrally distinct, information-rich images of single cells at a throughput of 5,000 cells per second. Yet often, cell populations are still studied using manual gating, a technique that has several dra...
Antimicrobial susceptibility is slow to determine, taking several days to fully impact treatment. This proof-of-concept study assessed the feasibility of using machine-learning techniques for analysis of data produced by the flow cytometer-assisted ...
Controlling the expression of genes is one of the key challenges of synthetic biology. Until recently fine-tuned control has been out of reach, particularly in eukaryotes owing to their complexity of gene regulation. With advances in machine learning...
Most applications of flow cytometry or cell sorting rely on the conjugation of fluorescent dyes to specific biomarkers. However, labeled biomarkers are not always available, they can be costly, and they may disrupt natural cell behavior. Label-free q...
Cytometry. Part A : the journal of the International Society for Analytical Cytology
Jul 16, 2019
We develop a single-mode fiber-based cytometer for the obtaining of two-dimensional (2D) light scattering patterns from static single cells. Anisotropy of the 2D light scattering patterns of single cells from ovarian cancer and normal cell lines is i...