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Genetic Therapy

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Low-Invasive Cell Injection based on Rotational Microrobot.

Advanced biosystems
The advancement of cell injections has created a need for accurate, efficient, and low-invasive injections. However, the conventional approaches to reduce cell damage during penetration, mainly optimization of micropipette tips and vision-based autom...

Future Vision 2020 and Beyond-5 Critical Trends in Eye Research.

Asia-Pacific journal of ophthalmology (Philadelphia, Pa.)
Ophthalmology has been at the forefront of many innovations in basic science and clinical research. The randomized prospective multicenter clinical trial, comparative clinical trials, the bench to beside development of diagnostic and therapeutic devi...

The retina revolution: signaling pathway therapies, genetic therapies, mitochondrial therapies, artificial intelligence.

Current opinion in ophthalmology
PURPOSE OF REVIEW: The aim of this article is to review and discuss the history, current state, and future implications of promising biomedical offerings in the field of retina.

Overcoming Immunological Challenges Limiting Capsid-Mediated Gene Therapy With Machine Learning.

Frontiers in immunology
A key hurdle to making adeno-associated virus (AAV) capsid mediated gene therapy broadly beneficial to all patients is overcoming pre-existing and therapy-induced immune responses to these vectors. Recent advances in high-throughput DNA synthesis, mu...

Machine learning based CRISPR gRNA design for therapeutic exon skipping.

PLoS computational biology
Restoring gene function by the induced skipping of deleterious exons has been shown to be effective for treating genetic disorders. However, many of the clinically successful therapies for exon skipping are transient oligonucleotide-based treatments ...

Recent Progress of Machine Learning in Gene Therapy.

Current gene therapy
With new developments in biomedical technology, it is now a viable therapeutic treatment to alter genes with techniques like CRISPR. At the same time, it is increasingly cheaper to perform whole genome sequencing, resulting in rapid advancement in ge...

Artificial Intelligence (AI)-Aided Structure Optimization for Enhanced Gene Delivery: The Effect of the Polymer Component Distribution (PCD).

ACS applied materials & interfaces
Gene therapy has emerged as a significant advancement in medicine in recent years. However, the development of effective gene delivery vectors, particularly polymer vectors, remains a significant challenge. Limited understanding of the internal struc...

Analysis on key parameters in subretinal injection facilitating a predictable and automated robot-assisted treatment in gene therapy.

The international journal of medical robotics + computer assisted surgery : MRCAS
BACKGROUND: Subretinal injection (SRI) has become an important surgical method for treating vitreoretinal diseases. Nevertheless, the optimisation of bleb formation in SRI, for the attainment of desired therapeutic outcomes, still requires further in...

Machine-learning-guided Directed Evolution for AAV Capsid Engineering.

Current pharmaceutical design
Target gene delivery is crucial to gene therapy. Adeno-associated virus (AAV) has emerged as a primary gene therapy vector due to its broad host range, long-term expression, and low pathogenicity. However, AAV vectors have some limitations, such as i...