BACKGROUND: As a promising neural stimulation technique, infrared neural stimulation (INS) has recently gained significant attention due to its ability to stimulate neuronal activities without needing exogenous agents. NIR light is absorbed by water ...
The application of algorithm-based single-cell imaging techniques can visualize and analyze cellular heterogeneity. However, algorithm-based single-cell imaging techniques are severely limited by the high workload required to label single-cell images...
Journal of the American Chemical Society
Jul 2, 2025
Fluorescence lifetime imaging microscopy (FLIM) is a powerful optical tool widely used in biomedical research to study changes in a sample's microenvironment. However, data collection and interpretation are often challenging, and traditional methods ...
Langmuir : the ACS journal of surfaces and colloids
Jun 3, 2025
The surface chemistry of nanomaterials, particularly the density of functional groups, governs their behavior in applications such as bioanalysis, bioimaging, and environmental impact studies. Here, we report a precise method to quantify carboxyl gro...
Fluorescence lifetime imaging microscopy (FLIM) has been widely used as an essential multiplexing and sensing tool in frontier fields such as materials science and life sciences. However, the accuracy of lifetime estimation is compromised by limited ...
We present an easy-to-use, nonlinear filter for effective background identification in fluorescence microscopy images with dense and low-contrast foreground. The pixel-wise filtering is based on comparison of the pixel intensity with the mean intensi...
The objective of optical super-resolution imaging is to acquire reliable sub-diffraction information on bioprocesses to facilitate scientific discovery. Structured illumination microscopy (SIM) is acknowledged as the optimal modality for live-cell su...
Proceedings of the National Academy of Sciences of the United States of America
May 27, 2025
Enhancing the reliability and reproducibility of optical microscopy by reducing specimen irradiance continues to be an important biotechnology target. As irradiance levels are reduced, however, the particle nature of light is heightened, giving rise ...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2025
Fluorescence microscopy is a key method for the visualization of cellular, subcellular, and molecular live-cell dynamics, enabling access to novel insights into mechanisms of health and disease. However, effects like phototoxicity, the fugitive natur...
The accuracy of assigning fluorophore identity and abundance, known as spectral unmixing, in biological fluorescence microscopy images remains a significant challenge due to the substantial overlap in emission spectra among fluorophores. In tradition...
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