AIMC Topic: Single-Cell Analysis

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X-scPAE: An explainable deep learning model for embryonic lineage allocation prediction based on single-cell transcriptomics revealing key genes in embryonic cell development.

Computers in biology and medicine
In single-cell transcriptomics research, accurately predicting cell lineage allocation and identifying differences between lineages are crucial for understanding cell differentiation processes and reducing early pregnancy miscarriages in humans. This...

Deciphering the role of cuproptosis in the development of intimal hyperplasia in rat carotid arteries using single cell analysis and machine learning techniques.

Scientific reports
UNLABELLED: This study aims to explore the regulatory role of cuproptosis in carotid intimal hyperplasia (IH), providing new insights into its pathophysiological mechanisms and potential diagnostic and therapeutic strategies.

Interpretable deep learning of single-cell and epigenetic data reveals novel molecular insights in aging.

Scientific reports
Deep learning (DL) and explainable artificial intelligence (XAI) have emerged as powerful machine-learning tools to identify complex predictive data patterns in a spatial or temporal domain. Here, we consider the application of DL and XAI to large om...

Single-Cell Array Enhanced Cell Damage Recognition Using Artificial Intelligence for Anticancer Drug Discovery.

Analytical chemistry
This work developed a cell damage recognition method based on single-cell arrays using an artificial intelligence tool. The method uses micropatterns (single-cell micropatches and microwells) to isolate each cell in an ordered array to minimize cell ...

Machine Learning Identification and Classification of Mitosis and Migration of Cancer Cells in a Lab-on-CMOS Capacitance Sensing Platform.

IEEE journal of biomedical and health informatics
Cell culture assays play a vital role in various fields of biology. Conventional assay techniques like immunohistochemistry, immunofluorescence, and flow cytometry offer valuable insights into cell phenotype and behavior. However, each of these techn...

Conditional similarity triplets enable covariate-informed representations of single-cell data.

BMC bioinformatics
BACKGROUND: Single-cell technologies enable comprehensive profiling of diverse immune cell-types through the measurement of multiple genes or proteins per individual cell. In order to translate immune signatures assayed from blood or tissue into powe...

Integration of single-cell and bulk RNA sequencing data using machine learning identifies oxidative stress-related genes LUM and PCOLCE2 as potential biomarkers for heart failure.

International journal of biological macromolecules
Oxidative stress (OS) is a pivotal mechanism driving the progression of cardiovascular diseases, particularly heart failure (HF). However, the comprehensive characterisation of OS-related genes in HF remains largely unexplored. In the present study, ...

Unravelling single-cell DNA replication timing dynamics using machine learning reveals heterogeneity in cancer progression.

Nature communications
Genomic heterogeneity has largely been overlooked in single-cell replication timing (scRT) studies. Here, we develop MnM, an efficient machine learning-based tool that allows disentangling scRT profiles from heterogenous samples. We use single-cell c...

A mechanism-informed deep neural network enables prioritization of regulators that drive cell state transitions.

Nature communications
Cells are regulated at multiple levels, from regulations of individual genes to interactions across multiple genes. Some recent neural network models can connect molecular changes to cellular phenotypes, but their design lacks modeling of regulatory ...

Deep learning powered single-cell clustering framework with enhanced accuracy and stability.

Scientific reports
Single-cell RNA sequencing (scRNA-seq) has revolutionized the field of cellular diversity research. Unsupervised clustering, a key technique in this exploration, allows for the identification of distinct cell types within a population. Graph-based de...