Use matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) to screen the specific mass peaks of carbapenem-resistant Klebsiella pneumoniae (CRKP), compare the differences in spectrum peaks between intestinal and b...
Carboxylesterases (CES) are pivotal enzymes in the hydrolysis of carboxylic esters, playing fundamental roles in both biological systems and biotechnological applications. This study investigates CES from the Amycolatopsis genus, characterized by its...
BACKGROUND: As bacterial pathogens develop increasing resistance to antibiotics, strategies targeting virulence factors (VFs) have emerged as a promising and effective approach for treating bacterial infections. Existing methods mainly relied on sequ...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Dec 25, 2024
The structures and activities of enzymes are influenced by pH of the environment. Understanding and distinguishing the adaptation mechanisms of enzymes to extreme pH values is of great significance for elucidating the molecular mechanisms and promoti...
Transcriptional factors (TFs) in bacteria play a crucial role in gene regulation by binding to specific DNA sequences, thereby assisting in the activation or repression of genes. Despite their central role, deciphering shape recognition of bacterial ...
Engineering enzyme-substrate binding pockets is the most efficient approach for modifying catalytic activity, but is limited if the substrate binding sites are indistinct. Here, we developed a 3D convolutional neural network for predicting protein-li...
Protein science : a publication of the Protein Society
Jun 1, 2024
Prokaryotic DNA binding proteins (DBPs) play pivotal roles in governing gene regulation, DNA replication, and various cellular functions. Accurate computational models for predicting prokaryotic DBPs hold immense promise in accelerating the discovery...
High-throughput DNA sequencing technologies decode tremendous amounts of microbial protein-coding gene sequences. However, accurately assigning protein functions to novel gene sequences remain a challenge. To this end, we developed FunGeneTyper, an e...
Methods in molecular biology (Clifton, N.J.)
Jan 1, 2022
Various Gram-negative bacteria use secretion systems to secrete effector proteins that manipulate host biochemical pathways to their benefit. We and others have previously developed machine-learning algorithms to predict novel effectors. Specifically...
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
Jan 1, 2022
Although natural products are rich sources for drug discovery, only a small percentage of natural products themselves have been approved for clinical use, thus it is necessary to modulate various properties, such as efficacy, toxicity, and metabolic ...
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