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Endopeptidases

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Enzymatic extraction and functional properties of phosphatidylcholine from chicken liver.

Poultry science
An environmentally sustainable method to extract phosphatidylcholine (PC) from chicken liver (PCCL) and its functional properties were studied. The extraction times, enzymatic hydrolysis time, the solid-liquid ratio as well as types of enzymes (prota...

Higher serum levels of fibroblast growth factor 21 in old patients with cachexia.

Nutrition (Burbank, Los Angeles County, Calif.)
OBJECTIVE: Fibroblast growth factor (FGF)21 is promptly induced by short fasting in animal models to regulate glucose and fat metabolism. Data on FGF21 in humans are inconsistent and FGF21 has not yet been investigated in old patients with cachexia, ...

Endolysin LysSA97 is synergistic with carvacrol in controlling Staphylococcus aureus in foods.

International journal of food microbiology
LysSA97 is an endolysin encoded by the bacteriophage SA97, the genome sequence of which has been recently revealed. LysSA97 has lytic activity against a variety of Staphylococcus strains that cause foodborne illness. In order to improve its potential...

Soft-sensing method based on FDLS-SVM in marine alkaline protease fermentation process.

Preparative biochemistry & biotechnology
To overcome the problem that soft-sensing model cannot be updated with the bioprocess changes, this article proposed a soft-sensing modeling method which combined fuzzy c-means clustering (FCM) algorithm with least squares support vector machine theo...

Soft Sensor Modeling Method Based on Improved KH-RBF Neural Network Bacteria Concentration in Marine Alkaline Protease Fermentation Process.

Applied biochemistry and biotechnology
Marine alkaline protease (MAP) fermentation is a complex multivariable, multi-coupled, and nonlinear process. Some unmeasured parameters will affect the quality of protease. Aiming at the problem that some parameters are difficult to be detected onli...

Higher-order structure formation using refined monomer structures of lipid raft markers, Stomatin, Prohibitin, Flotillin, and HflK/C-related proteins.

FEBS open bio
Currently, information on the higher-order structure of Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH)-domain proteins is limited. Briefly, the coordinate information (Refined PH1511.pdb) of the stomatin ortholog, PH1511 monomer, was obtained usi...

AlphaFold-Multimer predicts cross-kingdom interactions at the plant-pathogen interface.

Nature communications
Adapted plant pathogens from various microbial kingdoms produce hundreds of unrelated small secreted proteins (SSPs) with elusive roles. Here, we used AlphaFold-Multimer (AFM) to screen 1879 SSPs of seven tomato pathogens for interacting with six def...

Advances in the co-production of biosurfactant and other biomolecules: statistical approaches for process optimization.

Journal of applied microbiology
An efficient microbial conversion for simultaneous synthesis of multiple high-value compounds, such as biosurfactants and enzymes, is one of the most promising aspects for an economical bioprocess leading to a marked reduction in production cost. Alt...