AIMC Topic: Plasmids
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A machine learning-based approach for improving plasmid DNA production in Escherichia coli fed-batch fermentations.
Biotechnology journal
Jun 1, 2024
Artificial Intelligence (AI) technology is spearheading a new industrial revolution, which provides ample opportunities for the transformational development of traditional fermentation processes. During plasmid fermentation, traditional subjective pr...
PlasmidHunter: accurate and fast prediction of plasmid sequences using gene content profile and machine learning.
Briefings in bioinformatics
May 23, 2024
Plasmids are extrachromosomal DNA found in microorganisms. They often carry beneficial genes that help bacteria adapt to harsh conditions. Plasmids are also important tools in genetic engineering, gene therapy, and drug production. However, it can be...
Deeplasmid: deep learning accurately separates plasmids from bacterial chromosomes.
Nucleic acids research
Feb 22, 2022
Plasmids are mobile genetic elements that play a key role in microbial ecology and evolution by mediating horizontal transfer of important genes, such as antimicrobial resistance genes. Many microbial genomes have been sequenced by short read sequenc...
PlasGUN: gene prediction in plasmid metagenomic short reads using deep learning.
Bioinformatics (Oxford, England)
May 1, 2020
SUMMARY: We present the first tool of gene prediction, PlasGUN, for plasmid metagenomic short-read data. The tool, developed based on deep learning algorithm of multiple input Convolutional Neural Network, demonstrates much better performance when te...
Microfluidic cap-to-dispense (μCD): a universal microfluidic-robotic interface for automated pipette-free high-precision liquid handling.
Lab on a chip
Oct 9, 2019
Microfluidic devices have been increasingly used for low-volume liquid handling operations. However, laboratory automation of such delicate devices has lagged behind due to the lack of world-to-chip (macro-to-micro) interfaces. In this paper, we have...
PPR-Meta: a tool for identifying phages and plasmids from metagenomic fragments using deep learning.
GigaScience
Jun 1, 2019
BACKGROUND: Phages and plasmids are the major components of mobile genetic elements, and fragments from such elements generally co-exist with chromosome-derived fragments in sequenced metagenomic data. However, there is a lack of efficient methods th...
Enhancing fluorescent protein photostability through robot-assisted photobleaching.
Integrative biology : quantitative biosciences from nano to macro
Jul 16, 2018
Improving fluorescent proteins through the use of directed evolution requires robust techniques for screening large libraries of genetic variants. Here we describe an effective and relatively low-cost system for screening libraries of fluorescent pro...
[Research of enhanced green fluorescent protein gene transfer with ultrasound-mediated microbubble destruction in bone defects].
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery
Apr 15, 2017
OBJECTIVE: To investigate the effect of ultrasonic irradiation time on enhanced green fluorescent protein (EGFP) gene transfection efficiency and local tissue in bone defects using ultrasound-mediated microbubble destruction.
[Cloning, expression, and purification of c-di-AMP metabolism-related genes from Porphyromonas gingivalis].
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology
Dec 1, 2015
OBJECTIVE: To clone, express, and purify cyclic diadenosine monophosphate (c-di-AMP) metabolism-related genes from Porphyromonas gingivalis (P. gingivalis) ATCC33277.
[Construction of Recombinant Adenovirus Vector with Human/Mouse Interferon Gene and Its Transfection into Human Umbilical Cord Mesenchymal Stem Cells].
Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
Nov 1, 2015
OBJECTIVE: Construction and identification of recombinant adenovirus vector with human/mouse interferon (IFN-gamma) and effectively transfection into human umbilical cord mesenchymal stem cells (HUMSCs).
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