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Single-Domain Antibodies

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A novel nanobody and mimotope based immunoassay for rapid analysis of aflatoxin B1.

Talanta
Mimotopes could replace mycotoxins and their conjugates to develop immunoassay methods. The mimotopes obtained by phage display technology were mainly using monoclonal antibodies or polyclonal antibodies as targets. However, the mimotope of recombina...

[Construction and identification of nanobody phage display library targeting Middle East respiratory syndrome coronavirus].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Objective To construct a phage display library of specific nano-antibodies against the Middle East respiratory syndrome coronavirus (MERS-CoV) and apply it to the screening of neutralizing nano-antibodies. Methods MERS-CoV receptor-binding domain (RB...

Nanobody-based enzyme immunoassay for ochratoxin A in cereal with high resistance to matrix interference.

Talanta
A sensitive indirect competitive nanobody-based enzyme linked immunosorbent assay (Nb-ELISA) for ochratoxin A (OTA) with high resistance to cereal matrix interference was developed. Nanobodies against OTA (Nb15, Nb28, Nb32, Nb36) were expressed in E....

NanoNet: Rapid and accurate end-to-end nanobody modeling by deep learning.

Frontiers in immunology
Antibodies are a rapidly growing class of therapeutics. Recently, single domain camelid VHH antibodies, and their recognition nanobody domain (Nb) appeared as a cost-effective highly stable alternative to full-length antibodies. There is a growing ne...