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CONDITIONS OF PROOXIDANT ACTIVITY OF CISTUS AND POMEGRANATE POLYPHENOLS IN V79 CELL CULTURES.

Acta poloniae pharmaceutica
Polyphenol rich extracts obtained from cistus herb (Cistus incanus L.) and pomegranate peels (Punica granatunm L.) added at high concentrations (25-100 pg/mL) to V79 (Chinese hamster lung fibroblast) cell cultures, markedly increased intracellular le...

Alkaline phosphatase determines polyphosphate-induced mineralization in a cell-type independent manner.

Journal of bone and mineral metabolism
Polyphosphate [Poly(P)] has positive effects on osteoblast mineralization; however, the underlying mechanism remains unclear. In addition, it is unknown whether Poly(P) promotes mineralization in soft tissues. We investigated this by using various ce...

Use of uniform designs in combination with neural networks for viral infection process development.

Biotechnology progress
This work aimed to compare the predictive capacity of empirical models, based on the uniform design utilization combined to artificial neural networks with respect to classical factorial designs in bioprocess, using as example the rabies virus replic...

Artificial neural network associated to UV/Vis spectroscopy for monitoring bioreactions in biopharmaceutical processes.

Bioprocess and biosystems engineering
Currently, mammalian cells are the most utilized hosts for biopharmaceutical production. The culture media for these cell lines include commonly in their composition a pH indicator. Spectroscopic techniques are used for biopharmaceutical process moni...

Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production.

PloS one
The production of envelope glycoproteins (Envs) for use as HIV vaccines is challenging. The yield of Envs expressed in stable Chinese Hamster Ovary (CHO) cell lines is typically 10-100 fold lower than other glycoproteins of pharmaceutical interest. M...

Cell Segmentation Using a Similarity Interface With a Multi-Task Convolutional Neural Network.

IEEE journal of biomedical and health informatics
Even though convolutional neural networks (CNN) have been used for cell segmentation, they require pixel-level ground truth annotations. This paper proposes a multitask learning algorithm for cell detection and segmentation using CNNs. We use dot ann...

Prediction of hERG potassium channel blockage using ensemble learning methods and molecular fingerprints.

Toxicology letters
The human ether-a-go-go-related gene (hERG) encodes a tetrameric potassium channel called Kv11.1. This channel can be blocked by certain drugs, which leads to long QT syndrome, causing cardiotoxicity. This is a significant problem during drug develop...

Phase imaging with computational specificity (PICS) for measuring dry mass changes in sub-cellular compartments.

Nature communications
Due to its specificity, fluorescence microscopy has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit fluorescence microscopy's utility. Recently, it has been shown t...

Application of machine learning methods to pathogen safety evaluation in biological manufacturing processes.

Biotechnology progress
The production of recombinant therapeutic proteins from animal or human cell lines entails the risk of endogenous viral contamination from cell substrates and adventitious agents from raw materials and environment. One of the approaches to control su...