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Cryopreservation

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Effect of Timing of Postovulatory Insemination Relative to Human Chorionic Gonadotropin/Buserelin Treatment With 1 Straw of Frozen-Thawed Semen on Mare Fertility.

Journal of equine veterinary science
The reproductive management of mares for frozen semen artificial insemination (AI) can be costly and labor intensive. Predicting the exact time of ovulation can be challenging even when ovulation-inducing drugs are used. The main objective of this re...

Effect of Liquid Nitrogen Flushing of Extender on Seminal Antioxidant Profile of Murrah Buffalo during Cryopreservation.

Cryo letters
BACKGROUND: The dissolved oxygen in the extender may act as a source for the production of reactive oxygen species that may lead to reduced seminal antioxidant profile which in turn may be responsible for impaired frozen thawed sperm quality and fert...

Effect of Cholesterol-loaded Cyclodextrin on Membrane and Acrosome Status of Hariana Bull Sperm during Cryopreservation.

Cryo letters
BACKGROUND: The membrane and acrosomal integrity of sperm play a vital role in fertilization process; however they are compromised upon cryopreservation.

Effect of Using Two Cryopreservation Methods on Viability and Fertility of Frozen Stallion Sperm.

Journal of equine veterinary science
Studies involving different methods and techniques of cryopreservation and its interactions with the conception rates in artificial insemination (AI) programs are reported in the literature. This study evaluated the sperm kinetics, plasma membrane in...

Postthaw Addition of Autologous Seminal Plasma Improves Sperm Motion Characteristics in Fair and Poor Freezer Stallions.

Journal of equine veterinary science
During semen processing for cryopreservation, most seminal plasma is usually removed, and components with protective effects on sperm may be missing after thawing and within the female reproductive tract. The present study evaluated the effect of pos...

Cooling and Cryopreservation of Equine Platelet-Rich Plasma With Dimethyl Sulfoxide and Trehalose.

Journal of equine veterinary science
Equine platelet-rich plasma (PRP) has been used in horses to repair bone, articular and tendinous lesions, laminitis, and even endometritis. However, platelets have a very limited lifespan, which makes it difficult to prepare and use PRP, except in l...

Improvement in Semen Conservation of the Indigenous Czech Endangered Old Kladruber Horse: Special Focus on the Type of Extender and Packaging System.

Journal of equine veterinary science
The aim of our study was to investigate the effect of two freezing extenders and two packaging systems on motility, plasma membrane (PM) integrity, and the apoptotic status of frozen-thawed (F-T) spermatozoa of the endangered Old Kladruber stallions....

High spatially sensitive quantitative phase imaging assisted with deep neural network for classification of human spermatozoa under stressed condition.

Scientific reports
Sperm cell motility and morphology observed under the bright field microscopy are the only criteria for selecting a particular sperm cell during Intracytoplasmic Sperm Injection (ICSI) procedure of Assisted Reproductive Technology (ART). Several fact...