Genetics

Global microbial DNA signatures of temperature and nutrient limitation across ecosystems.

Microbial genomes continuously adapt to environmental conditions, but identifying universal signatures of adaptation remains challenging. Here we show that environmental temperature can be accurately predicted across ecosystems from DNA composition alone (R2 = 0.75), using tetranucleotide frequencies from 1,235 marine and soil metagenomes and a machine learning approach. This predictive signal was...

A novel adrenomedullin receptor signaling-based prognostic model predicts immunosuppressive microenvironment and informs therapeutic stratification in hepatocellular carcinoma: A retrospective bioinformatics study.

The adrenomedullin receptor signaling pathway plays a crucial role in tumor progression, yet its comprehensive implication in hepatocellular carcinoma (HCC) remains underexplored. This study aimed to develop and validate a multi-gene prognostic signature based on adrenomedullin receptor signaling-related genes (ARGs) and to elucidate its associations with clinicopathological features, immune micro...

Construction of a prognostic model using lactylation-related genes for predicting the prognosis of glioma patients.

Gliomas are the most lethal malignant tumors of the central nervous system, and their treatment continues to face serious challenges. Increasing evide...

CRISPR/Cas-based genome editing for enhancing grain quality traits in rice (Oryza sativa L.).

Rice is one of the most important food crops and feeds more than half of the world's population. Enhancing grain quality is currently a highly importa...

Proximity-confined DNA walking-nanozyme cascades for multiplexed urinary extracellular vesicle phenotyping and machine learning-assisted disease stratification.

Urinary extracellular vesicles (uEVs) provide noninvasive biomarkers for liquid biopsy owing to their ability to reflect disease-associated molecular ...

Integrative machine learning and single-nucleus transcriptomics reveal MAPK-centered neuronal responses underlying the potential Alzheimer's disease risk of GenX.

Hexafluoropropylene oxide-dimer acid (GenX or HFPO-DA) is a novel per- and polyfluoroalkyl substance developed as a replacement for legacy compounds, ...

Integrated population-based evidence, network toxicology, single-cell profiling, and in vitro studies elucidate mechanisms connecting PAHs to stroke.

Environmental exposure to polycyclic aromatic hydrocarbons (PAHs) has been associated with cardiovascular disease, yet the specific metabolites and ce...

FireMaster 550 Differentially Alters Gene Expression Underlying Synaptic Function in Amygdala of Prairie Voles After Gestational or Lactational Exposure.

Neurodevelopmental disorders often share similar behavioral diagnostic criteria including socioemotional and cognitive deficits. The prairie vole is a...

Disarming cGAS-STING hyperactivation in autoimmune and inflammatory diseases: Pathogenic mechanisms and emerging therapeutic strategies.

Aberrant activation of the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway has been increasingly recognized as a key dri...

ADRB2 Modulates Asbestos-Induced Pleural Mesothelioma Progression by Influencing IL-17/NF-κB-Related Inflammatory Signalling: An Integrated Network Toxicology, Multi-Omics and Experimental Study.

Pleural mesothelioma (PM) is a highly aggressive asbestos-related malignancy with poor prognosis and limited early diagnostic options. In this study, ...

Radiomics-Based AI for Predicting Neoadjuvant Immunochemotherapy Pathological Response in Non-Small Cell Lung Cancer: Systematic Review and Meta-Analysis.

BACKGROUND: Non-small cell lung cancer (NSCLC) remains the leading cause of cancer-related mortality worldwide. Accurate early prediction of response ...

Genome-wide machine learning analysis of anosmia and ageusia with COVID-19.

The COVID-19 pandemic has caused substantial worldwide disruptions in health, economy, and society, manifesting symptoms such as loss of smell (anosmi...

Local electrostatics governs tryptophan oxidation and enables rational stability engineering in antibodies.

Oxidation of tryptophan (Trp) residues in therapeutic antibody complementarity-determining regions (CDRs) can impair binding affinity, stability, and ...

Discovery of a potent TDP1 inhibitor through machine learning-driven predictive modeling combined with structure-based virtual screening and experimental validation.

Tyrosyl-DNA phosphodiesterase I (TDP1) repairs topoisomerase I (TOP1)-mediated DNA damage and is a promising anticancer target, particularly in combin...

CCT2 Promotes Immune Escape in Colorectal Cancer by Regulating the JAK1-STAT3-PD-L1 Axis.

BACKGROUND: Colorectal cancer (CRC) is a major global health burden. While immune checkpoint inhibitors have greatly advanced cancer therapy, their th...

Postoperative Nocardia cyriacigeorgica infection after glioblastoma resection: correlating metagenomic next-generation sequencing with conventional microbiology.

Postoperative nocardial infection after cranial surgery is rare and difficult to diagnose because Nocardia spp. grow slowly in conventional culture. M...

The role of "red flags" in the diagnostic work-up of hereditary transthyretin amyloidosis: a study using a machine-learning approach.

INTRODUCTION: Hereditary transthyretin amyloidosis (ATTRv) is a rare progressive, potentially life-threatening multisystem disorder caused by mutation...

Machine Learning Models Integrating CYP2C19 Genotypes for Long-Term Risk Prediction and Personalized Antiplatelet Therapy Selection in Myocardial Infarction.

Machine learning (ML) models integrating genetic and clinical data show promise for personalizing antiplatelet therapy after myocardial infarction (MI...

Enhancer Collapse Dictates PKHD1 Silencing and Centrosome-Mediated Chromosomal Instability and Metastasis in Clear Cell Renal Cell Carcinoma.

Chromosomal instability (CIN) drives clear cell renal cell carcinoma (ccRCC) progression, yet its upstream triggers remain elusive. To identify key dr...

Structural basis of enzymatic functional divergence in the PAL gene family of Salix brachista: Allosteric regulation and catalytic activity mediated by non-catalytic sites.

Phenylalanine ammonia-lyase (PAL) is the rate-limiting enzyme of the plant phenylpropanoid pathway, and its functional divergence is closely associate...