Genetics

CRISPR-Cas system: recent advancements in prompt diagnosis of high-risk HPV genotypes in cervical cancer.

INTRODUCTION: The CRISPR/Cas system has emerged as a highly versatile platform for diagnosing infectious diseases, particularly viral pathogens. Human papillomavirus (HPV) comprises of more than 200 types, with persistent infection by 14 high-risk genotypes recognized as the primary cause of cervical cancer worldwide. Early and accurate detection of these High-Risk HPV (HR-HPV) types is essential ...

A Thermogalvanic Cell Microneedle Patch for Integrated Diagnosis and Treatment of UV-Induced Early Skin Cancer.

Early stage skin cancer exhibits a high cure rate; however, conventional noninvasive screening suffers from diagnostic inaccuracies, and treatment reliant on postlesion surgery remains inherently passivity. This work develops an all-in-one platform by coupling microneedle (MN) patches with a lateral flow immunoassay (LFIA), seamlessly integrating self-powered biomarker extraction, multimodal detec...

Predictive modeling of gene expression and localization of DNA binding site using deep convolutional neural networks.

Despite the sequencing revolution, large swaths of the genomes sequenced to date lack any information about the arrangement of transcription factor bi...

Sonogenetics for Precision Medicine: A Focus on Immunoengineering and Genome Engineering.

Ultrasound can penetrate centimetres of soft tissue, focus energy with millimetre precision, and operate safely under real-time image guidance. Levera...

An end-to-end generalizable deep learning framework to comprehensively analyze transcriptional regulation.

Genome annotation currently requires performing dozens of molecular assays in hundreds of cell and tissue samples, an expensive endeavor which is impr...

Vietnamese Odonata: bridging global biodiversity, ecological, and conservation gaps in a changing world.

Vietnam is a global hotspot, hosting 493 Odonata species, with ~95% evaluated by the IUCN. Odonata species play vital ecological roles in aquatic and ...

Gene regulatory landscape dissected by single-cell four-omics sequencing.

Cellular diversity is governed not only by the transcriptome but also by multiple layers of epigenomic regulation, including nucleosome occupancy, chr...

Deep learning framework for predicting EGFR mutation status from H&E whole slide images in lung adenocarcinoma.

A novel deep learning-driven framework for improving lncRNA comprehensive annotation with LncADeep 2.0.

MOTIVATION: Long non-coding RNAs (lncRNAs) have emerged as crucial players in diverse physiological and pathological processes, yet the biological mec...

From prediction to precision: how immunopeptidomics advances neoantigen discovery.

T cell recognition of peptides presented by class I and II human leukocyte antigen (HLA) molecules is fundamental to cancer immunity and personalized ...

Bulk Microarray and Single-Cell Transcriptomic Analyses Reveal Bacterial Lipopolysaccharide-Related Biomarkers in Sepsis.

Sepsis, a life-threatening condition triggered by dysregulated host response to infection, poses significant global health challenges. Identifying lip...

StabLyzeGraph: High-throughput screening of combinatorial mutations using graph neural networks.

Engineering protein stability is a powerful strategy across biotechnology and medicine, supporting a broad range of applications such as atomic struct...

Enhanced Glioma Genotype Prediction Using CEST MRI With Full Z-Spectrum Input, Pixel-Level Learning, and Majority Voting.

Accurate glioma genotype prediction, such as the isocitrate dehydrogenase (IDH) mutation and O6-methylguanine-DNA methyltransferase (MGMT) promoter me...

Artificial intelligence in nongynecologic cytology: A systematic review of current research and commercial tools.

BACKGROUND: Nongynecologic (non-GYN) cytology plays an important role in cellular and molecular cancer diagnosis, although its use is limited by varia...

Usefulness of Artificial Intelligence for Surgical Support in Robot-assisted Distal Pancreatectomy: A Preliminary Case Report.

BACKGROUND/AIM: The incidence of postoperative complications in minimally-invasive surgery for pancreatic disease remains a concern. The application o...

Single-Cell Transcriptomic Analysis of Chemotherapy-Induced Changes in Osteosarcoma With a Pyroptosis-Related Gene-Based Prognostic Model.

Osteosarcoma, the most common primary malignant bone tumour, presents significant treatment challenges due to its complex tumour microenvironment and ...

A 3D morphogenetic blueprint for metastatic outgrowth in breast cancer.

The tissue-level processes underpinning metastatic outgrowth remain unclear. We combined single-cell RNA sequencing, spatial transcriptomics, and AI-s...

Learning multi-cellular representations of single-cell transcriptomics data enables characterization of patient-level disease states.

Single-cell RNA sequencing (scRNA-seq) is a prominent tool for studying human disease biology. The availability of massive scRNA-seq datasets and adva...

Testosterone-Sparing Treatment Strategies for Biochemically Recurrent Prostate Cancer After Radical Prostatectomy.

PURPOSE OF REVIEW: Biochemical recurrence (BCR) after radical prostatectomy occurs in up to one-third of patients and increases the risk of metastasis...

Seeing and Feeling DNA Methylation: Single-Molecule Biophysics Meets Machine Learning.

DNA methylation at 5-methylcytosine (5mC) is crucial for embryonic development and cellular function, while aberrant patterns strongly drive disease o...