Pathology

CRISPR/Cas12a: A Comprehensive Review from Structural Foundations to Applications in Nucleic Acid Precision Detection.

CRISPR/Cas12a technology, characterized by its distinctive trans-cleavage activity, has evolved beyond its gene-editing function to emerge as a powerful tool for molecular detection. This review systematically delineates its structural foundation and molecular mechanism, with a focus on how the technology converts specific nucleic acid recognition into cascade signal amplification. Its application...

Molecularly Engineered SERS Platform with Microturbulence-Enhanced Electrohydrodynamics for Multiplexed Profiling of Lung Cancer ctDNA.

Precise profiling of multiple circulating tumor DNA (ctDNA) is hindered by the challenge of resolving single-nucleotide variants (SNVs) and their trace abundance. Herein, we present an integrated SERS platform that combines rationally molecularly engineered nanotags with a novel microturbulent electrofluidic interface for multiplexed SNV-level profiling of lung cancer ctDNAs. In particular, the SE...

Technical Description of the Mini Far-Lateral Approach: A Refined, Stability-Preserving Corridor to the Petroclival Region.

BACKGROUND AND OBJECTIVES: The far-lateral approach remains essential for accessing ventral and ventrolateral lesions of the craniovertebral junction....

Enhancing drug synergy in malignant diseases with deep architecture optimization algorithms.

Malignant diseases remain one of the leading causes of death globally. Drug synergy has emerged as an effective approach for treating malignancy, offe...

Artificial Intelligence and Nasal Cytology: Toward a Comprehensive Cytological Endotyping of Rhinitis.

Comparison of breast ultrasound image classification accuracy between convolutional neural networks and human experts using multicenter external validation cohort data.

PURPOSE: In recent years, much research has been conducted on ultrasound diagnosis of breast tumors using convolutional neural networks (CNNs). While ...

Contemporary Indications and Technical Limits of Transoral Laser Microsurgery for Laryngeal Squamous Cell Carcinoma.

Transoral laser microsurgery (TOLMS) is an established organ-preserving treatment for early-stage and selected intermediate-stage laryngeal squamous c...

A Deep Learning-Driven Framework Integrating Organoid-Based Functional Validation Identifies Universal Neoantigens from Recurrent Glioma Mutations.

Glioblastoma (GBM) is the most common malignant intracranial tumor in adults, with a median survival of only 16-20 months. Neoantigen therapy has show...

Prostate cancer tissue mapping and stratification using DRAQ5 and Eosin fluorescent labels integrated with AI classification and segmentation algorithms.

BACKGROUND: Fluorescent microscopy using the DRAQ5 and Eosin probes has been shown in the literature to be capable of producing rapid tissue character...

Immune gene MMP9 as a potential key mediator in TCDD exposure-associated atherosclerosis: An integrated study based on network toxicology and experimental validation.

BACKGROUND: 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a highly toxic, recalcitrant persistent organic pollutant with ubiquitous environmental dist...

Ultrasound Localization Microscopy Learned from power doppler by uncertainty frequency density estimation and semantic consistency awareness.

Ultrasound Localization Microscopy (ULM) achieves micron-level vascular visualization beyond the resolution of conventional ultrasound imaging by trac...

Unlocking azobenzene isomerization mechanisms via an LLM agent-driven workflow integrating simulation, experiment, and machine learning.

Bridged azobenzene derivatives are key photo-responsive molecular switches. However, probing and interpreting their microscopic Z ↔ E isomerization me...

Human microphysiological systems of aging recreate the in vivo process expediting evaluation of anti-geronic strategies.

The search for biological mechanisms of human aging is stalled by a lack of suitable models, and it remains unknown whether and to what degree rejuven...

Advanced imaging techniques for tumor intraoperative navigation imaging.

Cancer remains a leading cause of mortality and morbidity, with surgical resection being a fundamental element of treatment. However, early detection ...

Finding the most promising indications for novel treatments in oncology.

Tackling cancer with effective therapies is a major challenge our society faces with significant costs and complexities of designing and validating tr...

AI-Assisted Tissue Classification in Chironomus riparius: A Potential Tool for Ecotoxicological Studies.

Histological techniques are valuable tools in various biological and medical disciplines. They have proven particularly valuable in ecotoxicological s...

An interpretable machine learning model combining MRI-DKI habitat radiomic features and clinical biomarkers for noninvasive prediction of lymphatic metastasis in rectal cancer: a prospective study.

OBJECTIVE: Tumor heterogeneity exerts a significant influence on lymphovascular invasion (LVI) and lymph node metastasis (LNM) in rectal cancer (RC), ...

A Multimodal Attention Fusion-Based Model for Pathological Voice Detection.

Pathological voice detection provides a non-invasive and cost-effective approach for the early screening of laryngeal disorders. However, most existin...

Efficient machine learning models leveraging DCE‑MRI morphological and dynamic features allow accurate breast lesion classification.

We propose an ensemble learning approach to classify malignant versus benign breast lesions leveraging morphological and dynamic features derived from...

[Comprehensive diagnosis and treatment of distal biliary strictures: multidisciplinary collaboration and precision surgical strategies].

Distal biliary strictures (DBS) are a common and complex clinical problem in pancreaticobiliary diseases,with etiologies involving multiple factors su...