Sheng wu gong cheng xue bao = Chinese journal of biotechnology
May 25, 2021
As an important model industrial microorganism, Escherichia coli has been widely used in pharmaceutical, chemical industry and agriculture. In the past 30 years, a variety of new strategies and techniques, including artificial intelligence, gene edit...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Mar 25, 2021
Industrial microorganisms and their products are widely used in various fields such as industry, agriculture, and medicine, which play a pivotal role in economy. Efficient industrial strains are the key to improve production efficiency, and advanced ...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Sep 25, 2019
With the development of mass spectrometry technologies and bioinformatics analysis algorithms, disease research-driven human proteome project (HPP) is advancing rapidly. Protein biomarkers play critical roles in clinical applications and the biomarke...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Apr 25, 2019
In order to provide a theoretical basis for better understanding the function and properties of proteins, we proposed a simple and effective feature extraction method for protein sequences to determine the subcellular localization of proteins. First,...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Dec 25, 2017
Pectate lyase is widely applied in ramie degumming and fabric bioscouring in the textile industry. Compared to conventional processes that involve high alkaline and high temperature treatment, enzyme based treatments have significant advantages in fi...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Dec 25, 2016
The aim of this study is to prepare and characterize cardiac troponin T (cTnT) monoclonal antibodies (mAb), and further develop a chemiluminescence quantitative detection assay for cTnT. BALB/c mice were immunized with recombinant cTnT antigen, and s...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Oct 25, 2016
Deficient activity of endo-1,4-beta-glucanase II (Cel5A) secreted by Trichoderma reesei is one of the challenges involved in effective cellulase saccharification of cellulosic substrates. Therefore, we expressed Cel5A in Pichia pastoris by constructi...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Dec 1, 2015
The 1,095 bp gene encoding peroxidase from Coprinus cinereus was synthesized and integrated into the genome of Pichia pastoris with a highly inducible alcohol oxidase. The recombinant CiP (rCiP) fused with the a-mating factor per-pro leader sequence ...
Sheng wu gong cheng xue bao = Chinese journal of biotechnology
Nov 1, 2015
A method for quantitation of collagen was established by detecting marker peptide with high performance liquid chromatography-mass spectrometry (HPLC-MS). Theoretical marker peptides were selected by sequence comparison. Bovine collagen type I was di...