AIMC Topic: Animals, Genetically Modified

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High-throughput brain activity mapping and machine learning as a foundation for systems neuropharmacology.

Nature communications
Technologies for mapping the spatial and temporal patterns of neural activity have advanced our understanding of brain function in both health and disease. An important application of these technologies is the discovery of next-generation neurotherap...

Determination of total plasma oxysterols by enzymatic hydrolysis, solid phase extraction and liquid chromatography coupled to mass-spectrometry.

Journal of pharmaceutical and biomedical analysis
The potential use of cholesterol esterases was tested to avoid alkaline hydrolysis for cleavage of plasma esterified oxysterols. The enzymatic hydrolysis was optimized by testing two sources of enzyme-Pseudomonas and bovine pancreas, presence of surf...

Transcriptomes of lineage-specific Drosophila neuroblasts profiled by genetic targeting and robotic sorting.

Development (Cambridge, England)
A brain consists of numerous distinct neurons arising from a limited number of progenitors, called neuroblasts in Drosophila. Each neuroblast produces a specific neuronal lineage. To unravel the transcriptional networks that underlie the development ...

Computer-Assisted Transgenesis of Caenorhabditis elegans for Deep Phenotyping.

Genetics
A major goal in the study of human diseases is to assign functions to genes or genetic variants. The model organism Caenorhabditis elegans provides a powerful tool because homologs of many human genes are identifiable, and large collections of geneti...

The interaction of UBR4, LRP1, and OPHN1 in refractory epilepsy: Drosophila model to investigate the oligogenic effect on epilepsy.

Neurobiology of disease
Refractory epilepsy is an intractable neurological disorder that can be associated with oligogenic/polygenic etiologies. Through trio-based whole-exome sequencing analysis, we identified a clinical case of refractory epilepsy with three candidate gen...

High-throughput genetic manipulation of multicellular organisms using a machine-vision guided embryonic microinjection robot.

Genetics
Microinjection is a technique used for transgenesis, mutagenesis, cell labeling, cryopreservation, and in vitro fertilization in multiple single and multicellular organisms. Microinjection requires specialized skills and involves rate-limiting and la...

Toward a living soft microrobot through optogenetic locomotion control of .

Science robotics
Learning from the locomotion of natural organisms is one of the most effective strategies for designing microrobots. However, the development of bioinspired microrobots is still challenging because of technical bottlenecks such as design and seamless...

Investigating the anti-angiogenic effects of Fufang Kushen Injection in combination with cisplatin using a zebrafish model.

Pakistan journal of pharmaceutical sciences
The Traditional Chinese Medicine formula Fufang Kushen Injection (FKI) has demonstrated potential to enhance the efficacy and reduce the toxicity of the chemotherapeutic drug cisplatin. However, there is insufficient evidence to determine whether the...