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Antibodies, Monoclonal

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Development challenges of high concentration monoclonal antibody formulations.

Drug discovery today. Technologies
High concentration monoclonal antibody drug products represent a special segment of biopharmaceuticals. In contrast to other monoclonal antibody products, high concentration monoclonal antibodies are injected subcutaneously helping increase patient c...

Single B cell technologies for monoclonal antibody discovery.

Trends in immunology
Monoclonal antibodies (mAbs) are often selected from antigen-specific single B cells derived from different hosts, which are notably short-lived in ex vivo culture conditions and hence, arduous to interrogate. The development of several new technique...

In silico proof of principle of machine learning-based antibody design at unconstrained scale.

mAbs
Generative machine learning (ML) has been postulated to become a major driver in the computational design of antigen-specific monoclonal antibodies (mAb). However, efforts to confirm this hypothesis have been hindered by the infeasibility of testing ...

Progress and challenges for the machine learning-based design of fit-for-purpose monoclonal antibodies.

mAbs
Although the therapeutic efficacy and commercial success of monoclonal antibodies (mAbs) are tremendous, the design and discovery of new candidates remain a time and cost-intensive endeavor. In this regard, progress in the generation of data describi...

Machine learning prediction of antibody aggregation and viscosity for high concentration formulation development of protein therapeutics.

mAbs
Machine learning has been recently used to predict therapeutic antibody aggregation rates and viscosity at high concentrations (150 mg/ml). These works focused on commercially available antibodies, which may have been optimized for stability. In this...

Machine Learning Analysis Provides Insight into Mechanisms of Protein Particle Formation Inside Containers During Mechanical Agitation.

Journal of pharmaceutical sciences
Container choice can influence particle generation within protein formulations. Incompatibility between proteins and containers can manifest as increased particle concentrations, shifts in particle size distributions and changes in particle morpholog...

Prediction of HIV sensitivity to monoclonal antibodies using aminoacid sequences and deep learning.

Bioinformatics (Oxford, England)
MOTIVATION: Knowing the sensitivity of a viral strain versus a monoclonal antibody is of interest for HIV vaccine development and therapy. The HIV strains vary in their resistance to antibodies, and the accurate prediction of virus-antibody sensitivi...

An automated, low volume, and high-throughput analytical platform for aggregate quantitation from cell culture media.

Journal of chromatography. A
High throughput screening methods have driven a paradigm shift in biopharmaceutical development by reducing the costs of good manufactured (COGM) and accelerate the launch to market of novel drug products. Scale-down cell culture systems such as shak...

Multienzyme deep learning models improve peptide de novo sequencing by mass spectrometry proteomics.

PLoS computational biology
Generating and analyzing overlapping peptides through multienzymatic digestion is an efficient procedure for de novo protein using from bottom-up mass spectrometry (MS). Despite improved instrumentation and software, de novo MS data analysis remains ...

Data-driven prediction models for forecasting multistep ahead profiles of mammalian cell culture toward bioprocess digital twins.

Biotechnology and bioengineering
Recently, the advancement in process analytical technology and artificial intelligence (AI) has enabled the generation of enormous culture data sets from biomanufacturing processes that produce various recombinant therapeutic proteins (RTPs), such as...